Summary of ssbd-repos-000349

Name
URL
DOI

Title
High-dimensional and large-scale phenotyping of yeast mutants
Description

Image and morphological data for high-dimensional and large-scale phenotyping of yeast mutants

Submited Date
2024-05-16
Release Date
2024-07-12
Updated Date
-
License
Funding information
This work was supported by the Institute for Bioinformatics and Research and Development of the Japan Science and Technology Corporation.
File formats
jpg, tsv, txt, xls, xlsx
Data size
45.3 GB

Organism
Saccharomyces cerevisiae
Strain
-
Cell Line
-
Genes
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Proteins
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GO Molecular Function (MF)
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GO Biological Process (BP)
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GO Cellular Component (CC)
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Study Type
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Imaging Methods
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Method Summary

See details in Ohya et. al. (2005) Proceedings of the National Academy of Sciences.

Related paper(s)

Yoshikazu Ohya, Jun Sese, Masashi Yukawa, Fumi Sano, Yoichiro Nakatani, Taro L Saito, Ayaka Saka, Tomoyuki Fukuda, Satoru Ishihara, Satomi Oka, Genjiro Suzuki, Machika Watanabe, Aiko Hirata, Miwaka Ohtani, Hiroshi Sawai, Nicolas Fraysse, Jean-Paul Latge, Jean M Francois, Markus Aebi, Seiji Tanaka, Sachiko Muramatsu, Hiroyuki Araki, Kintake Sonoike, Satoru Nogami, Shinichi Morishita (2005) High-dimensional and large-scale phenotyping of yeast mutants., Proceedings of the National Academy of Sciences of the United States of America, Volume 102, Number 52, pp. 19015-20

Published in 2005 Dec 27 (Electronic publication in Dec. 19, 2005, midnight )

(Abstract) One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.
(MeSH Terms)

Contact(s)
Yoshikazu Ohya
Organization(s)
University of Tokyo , Department of Integrated Biosciences, Graduate School of Frontier Sciences, , Laboratory of Signal Transduction
Image Data Contributors
Quantitative Data Contributors

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