Summary of ssbd-repos-000227

SSBD:database
URL

Name
URL
DOI

Title
Raman imaging in HeLa cells treated with D-alkyne-tagged C18FAs
Description

Two-color Raman imaging of similar small molecules in living cells by using D-/H-alkynes as Raman tags

Submited Date
2022-04-18
Release Date
2022-04-18
Updated Date
-
License
Funding information
-
File formats
bmp
Data size
4.4 MB

Organism
H. sapiens
Strain
HeLa cell
Cell Line
-
Genes
-
Proteins
-

GO Molecular Function (MF)
-
GO Biological Process (BP)
fatty acids localization, EdU localization
GO Cellular Component (CC)
lipids, nucleus
Study Type
Development of new Raman tag
Imaging Methods
Spontaneous Raman microscopy

Method Summary

Egoshi S, Dodo K, Ohgane K, Sodeoka M. Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules. Org Biomol Chem. 2021 Oct 14;19(38), 8232-8236. doi.org/10.1039/D1OB01479J. PMID: 34528645.

Related paper(s)

Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236

Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )

(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
(MeSH Terms)

Contact(s)
Kosuke Dodo, Mikiko Sodeoka
Organization(s)
RIKEN , Cluster for Pioneering Research , Synthetic organic chemistry laboratory
Image Data Contributors
Syusuke Egoshi
Quantitative Data Contributors

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