Two-color Raman imaging of similar small molecules in living cells by using D-/H-alkynes as Raman tags
Egoshi S, Dodo K, Ohgane K, Sodeoka M. Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules. Org Biomol Chem. 2021 Oct 14;19(38), 8232-8236. doi.org/10.1039/D1OB01479J. PMID: 34528645.
Syusuke Egoshi, Kosuke Dodo, Kenji Ohgane, Mikiko Sodeoka (2021) Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules., Organic & biomolecular chemistry, Volume 19, Number 38, pp. 8232-8236
Published in 2021 Oct 6 (Electronic publication in Oct. 6, 2021, midnight )
(Abstract) Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.(MeSH Terms)