Summary of ssbd-repos-000170


SSBD:database
SSBD:database URL

SSBD:repository
SSBD:repository URL
DOI
Title
Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions
Description

Inter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial–fibroblast, epithelial–muscle and epithelial–nerve interactions. The epithelial–fibroblast interface, namely, hair germ–dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin alpha5, is required for hair cycle regulation and hair germ–dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.

Submited Date
2021-03-30
Release Date
2021-05-11
Updated Date
-
License
Data size
2.9 GB
Data formats
Lif

Organism
Mus musculus
Strain
C57BL/6
Cell Line
-
Molecular Function (MF)
extracellular matrix structural constituent
Biological Process (BP)
extracellular matrix organization
Cellular Component (CC)
extracellular matrix
Study Type
extracellular matrix, basement membrane, cell adhesion
Imaging Methods
confocal microscopy

Method Summary

See details in Tsutsui, K., Machida, H., Nakagawa, A. et al. Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions. Nat Commun 12, 2577 (2021).

Related paper(s)

Ko Tsutsui, Hiroki Machida, Asako Nakagawa, Kyungmin Ahn, Ritsuko Morita, Kiyotoshi Sekiguchi, Jeffrey H Miner, Hironobu Fujiwara (2021) Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions., Nature communications, Volume 12, Number 1, pp. 2577

Published in 2021 May 10 (Electronic publication in May 10, 2021, midnight )

(Abstract) Inter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial-fibroblast, epithelial-muscle and epithelial-nerve interactions. The epithelial-fibroblast interface, namely, hair germ-dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin alpha5, is required for hair cycle regulation and hair germ-dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.
(MeSH Terms)

Contact(s)
Hironobu Fujiwara
Organization(s)
RIKEN , Center for Biosystems Dynamics Research , Laboratory for Tissue Microenvironment
Image Data Contributors
Ko Tsutsui, Hiroki Machida, Asako Nakagawa
Quantitative Data Contributors
Ko Tsutsui

Download files