Summary of 82-Ikeda-GeneExp

SSBD:database
SSBD:database URL
Title
Immunofluorescence images of different type neural progenitor cells (NPCs)
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
9 ( Image datasets: 9, Quantitative data datasets: 0 )
Size of Datasets
50.4 MB ( Image datasets: 50.4 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Gene symbol(s)
Actb
Protein name(s)
Mkl1
Reporter(s)
DAPI, anti-Mkl1

Datatype
gene expression
Molecular Function (MF)
Biological Process (BP)
gene expression
Cellular Component (CC)
nucleus
Biological Imaging Method
-
XYZ Scale
XY: 353 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
FluorescenceMicroscope
Acquisition mode
WideField
Contrast method
Fluorescence
Microscope model
Olympus IX73
Detector model
Olympus DP73
Objective model
Olympus LUCPlanFL N 40x
Filter set
Olympus IX3-RFACS

Related paper(s)

Takashi Ikeda, Takafusa Hikichi, Hisashi Miura, Hirofumi Shibata, Kanae Mitsunaga, Yosuke Yamada, Knut Woltjen, Kei Miyamoto, Ichiro Hiratani, Yasuhiro Yamada, Akitsu Hotta, Takuya Yamamoto, Keisuke Okita, Shinji Masui (2018) Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs., Nature communications, Volume 9, Number 1, pp. 1387

Published in 2018 Apr 11 (Electronic publication in April 11, 2018, midnight )

(Abstract) Multicellular organisms consist of multiple cell types. The identity of these cells is primarily maintained by cell-type-specific gene expression programs; however, mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease beta-actin monomer quantity result in the nuclear accumulation of Mkl1 and the activation of Srf, which downregulate cell-type-specific genes and alter the epigenetics of regulatory regions and chromatin organization. Mice overexpressing Srf exhibit various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
(MeSH Terms)

Contact
Takashi Ikeda, Keisuke Okita, Shinji Masui , Kyoto University , Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA) , Okita Lab
Contributors
Takashi Ikeda, Takafusa Hikichi, Hisashi Miura, Hirofumi Shibata, Kanae Mitsunaga, Yosuke Yamada, Knut Woltjen, Kei Miyamoto, Ichiro Hiratani, Yasuhiro Yamada, Akitsu Hotta, Takuya Yamamoto, Keisuke Okita, Shinji Masui


Dataset List of 82-Ikeda-GeneExp

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4055
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4056
Datast ID ActbKO1_DAPI
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4057
Datast ID ActbKO1_MkI1
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4058
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4059
Datast ID ActbKO2_DAPI
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4060
Datast ID ActbKO2_MkI1
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4061
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4062
Datast ID control_DAPI
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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# 4063
Datast ID control_Mkl1
Dataset Kind Image data
Dataset Size 5.6 MB
4D view
SSBD:OMERO
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