Summary of 74-Bansod-NSCellHes5

SSBD:database
SSBD:database URL
Title
Neurogenesis and gliogenesis in WT and Hes5-overexpressing Tg brain
Description
-
Relase date
2018-11-14
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
64 ( Image datasets: 64, Quantitative data datasets: 0 )
Size of Datasets
124.7 MB ( Image datasets: 124.7 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Strain(s)
NIH3T3
Protein name(s)
Ctip2, Cux1, EduU, Ki67, Pax6, Tbr1, Tbr2, Tuj1
Reporter(s)
DAPI, NeuN, cleaved-caspase3

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
gliogenesis, neurogenesis
Cellular Component (CC)
-
Biological Imaging Method
-
XYZ Scale
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T scale
-

Image Acquisition
Experiment type
Immunocytochemistry
Microscope type
FluorescenceMicroscope
Acquisition mode
BrightField
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Shama Bansod, Ryoichiro Kageyama, Toshiyuki Ohtsuka (2017) Hes5 regulates the transition timing of neurogenesis and gliogenesis in mammalian neocortical development., Development (Cambridge, England), Volume 144, Number 17, pp. 3156-3167

Published in 2017 Sep 1

(Abstract) During mammalian neocortical development, neural stem/progenitor cells (NSCs) sequentially give rise to deep layer neurons and superficial layer neurons through mid- to late-embryonic stages, shifting to gliogenic phase at perinatal stages. Previously, we found that the Hes genes inhibit neuronal differentiation and maintain NSCs. Here, we generated transgenic mice that overexpress Hes5 in NSCs of the central nervous system, and found that the transition timing from deep to superficial layer neurogenesis was shifted earlier, while gliogenesis precociously occurred in the developing neocortex of Hes5-overexpressing mice. By contrast, the transition from deep to superficial layer neurogenesis and the onset of gliogenesis were delayed in Hes5 knockout (KO) mice. We found that the Hmga genes (Hmga1/2) were downregulated in the neocortical regions of Hes5-overexpressing brain, whereas they were upregulated in the Hes5 KO brain. Furthermore, we found that Hes5 expression led to suppression of Hmga1/2 promoter activity. These results suggest that Hes5 regulates the transition timing between phases for specification of neocortical neurons and between neurogenesis and gliogenesis, accompanied by alteration in the expression levels of Hgma genes, in mammalian neocortical development.
(MeSH Terms)

Contact
Toshiyuki Ohtsuka , Kyoto University , Institute for Frontier Life and Medical Sciences
Contributors
Shama Bansod, Ryoichiro Kageyama, Toshiyuki Ohtsuka


Dataset List of 74-Bansod-NSCellHes5

#
Dataset ID
Kind
Size
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# 3949
Dataset Kind Image data
Dataset Size 4.0 MB
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# 3950
Dataset Kind Image data
Dataset Size 4.0 MB
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# 3951
Dataset Kind Image data
Dataset Size 4.0 MB
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# 3952
Dataset Kind Image data
Dataset Size 1.1 MB
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# 3953
Dataset Kind Image data
Dataset Size 3.0 MB
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# 3954
Dataset Kind Image data
Dataset Size 1.9 MB
4D view
SSBD:OMERO
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# 3955
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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# 3956
Dataset Kind Image data
Dataset Size 2.7 MB
4D view
SSBD:OMERO
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# 3957
Dataset Kind Image data
Dataset Size 3.0 MB
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SSBD:OMERO
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# 3958
Dataset Kind Image data
Dataset Size 3.0 MB
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SSBD:OMERO
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# 3959
Dataset Kind Image data
Dataset Size 927.4 KB
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# 3960
Dataset Kind Image data
Dataset Size 1.5 MB
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# 3961
Dataset Kind Image data
Dataset Size 1.3 MB
4D view
SSBD:OMERO
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# 3962
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 3963
Dataset Kind Image data
Dataset Size 1.1 MB
4D view
SSBD:OMERO
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# 3964
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 3965
Dataset Kind Image data
Dataset Size 2.1 MB
4D view
SSBD:OMERO
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# 3966
Dataset Kind Image data
Dataset Size 1.4 MB
4D view
SSBD:OMERO
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# 3967
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 3968
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 3969
Dataset Kind Image data
Dataset Size 857.1 KB
4D view
SSBD:OMERO
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# 3970
Datast ID Fig1I_E15.5Tg_NeuN
Dataset Kind Image data
Dataset Size 852.9 KB
4D view
SSBD:OMERO
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# 3971
Dataset Kind Image data
Dataset Size 1.1 MB
4D view
SSBD:OMERO
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# 3972
Datast ID Fig1I_E15.5WT_NeuN
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 3973
Datast ID Fig1I_E13.5Tg_NeuN
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 3974
Datast ID Fig1I_E17.5Tg_NeuN
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 3975
Datast ID Fig1I_P0Tg_NeuN
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 3976
Datast ID Fig1I_E13.5WT_NeuN
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 3977
Datast ID Fig1I_E17.5WT_NeuN
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 3978
Datast ID Fig1I_P0WT_NeuN
Dataset Kind Image data
Dataset Size 2.1 MB
4D view
SSBD:OMERO
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# 3979
Dataset Kind Image data
Dataset Size 1.4 MB
4D view
SSBD:OMERO
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# 3980
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 3981
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 3982
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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# 3983
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 3984
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 3985
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 3986
Dataset Kind Image data
Dataset Size 3.0 MB
4D view
SSBD:OMERO
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# 3987
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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# 3988
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 3989
Dataset Kind Image data
Dataset Size 2.3 MB
4D view
SSBD:OMERO
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# 3990
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 3991
Dataset Kind Image data
Dataset Size 1.5 MB
4D view
SSBD:OMERO
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# 3992
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 3993
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 3994
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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# 3995
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 3996
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 3997
Dataset Kind Image data
Dataset Size 1.9 MB
4D view
SSBD:OMERO
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# 3998
Dataset Kind Image data
Dataset Size 1.9 MB
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SSBD:OMERO
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# 3999
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 4000
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 4001
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 4002
Dataset Kind Image data
Dataset Size 1.7 MB
4D view
SSBD:OMERO
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# 4003
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
SSBD:OMERO
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# 4004
Dataset Kind Image data
Dataset Size 1.6 MB
4D view
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# 4005
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 4006
Dataset Kind Image data
Dataset Size 1.8 MB
4D view
SSBD:OMERO
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# 4007
Dataset Kind Image data
Dataset Size 775.1 KB
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# 4008
Dataset Kind Image data
Dataset Size 775.1 KB
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# 4009
Dataset Kind Image data
Dataset Size 2.1 MB
4D view
SSBD:OMERO
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# 4010
Dataset Kind Image data
Dataset Size 2.4 MB
4D view
SSBD:OMERO
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# 4011
Dataset Kind Image data
Dataset Size 1.9 MB
4D view
SSBD:OMERO
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# 4012
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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