Summary of 281-Kogure-EmbryoDev

SSBD:database URL
Time-lapse images of WT and Admp MO embryos from late neurula to late tailbud stage.
Relase date
Updated date
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
470.3 MB ( Image datasets: 470.3 MB, Quantitative data datasets: 0 bytes )

Ciona robusta
Nile Blue B

Molecular Function (MF)
Biological Process (BP)
embryo development
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
X scale
4.78 micrometer/pixel
Y scale
4.78 micrometer/pixel
Z scale
T scale
1 minute of time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Related paper(s)

Yuki S Kogure, Hiromochi Muraoka, Wataru C Koizumi, Raphael Gelin-Alessi, Benoit Godard, Kotaro Oka, Carl-Philipp Heisenberg, Kohji Hotta (2022) Admp regulates tail bending by controlling ventral epidermal cell polarity via phosphorylated myosin localization in Ciona., Development (Cambridge, England), Volume 149, Number 21

Published in 2022 Nov 1 (Electronic publication in Oct. 19, 2022, midnight )

(Abstract) Ventral tail bending, which is transient but pronounced, is found in many chordate embryos and constitutes an interesting model of how tissue interactions control embryo shape. Here, we identify one key upstream regulator of ventral tail bending in embryos of the ascidian Ciona. We show that during the early tailbud stages, ventral epidermal cells exhibit a boat-shaped morphology (boat cell) with a narrow apical surface where phosphorylated myosin light chain (pMLC) accumulates. We further show that interfering with the function of the BMP ligand Admp led to pMLC localizing to the basal instead of the apical side of ventral epidermal cells and a reduced number of boat cells. Finally, we show that cutting ventral epidermal midline cells at their apex using an ultraviolet laser relaxed ventral tail bending. Based on these results, we propose a previously unreported function for Admp in localizing pMLC to the apical side of ventral epidermal cells, which causes the tail to bend ventrally by resisting antero-posterior notochord extension at the ventral side of the tail.
(MeSH Terms)

Kohji Hotta, Carl-Philipp Heisenberg , Keio University, Institute of Science and Technology Austria , Faculty of Science and Technology , Department of Biosciences and Informatics

Dataset List of 281-Kogure-EmbryoDev

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# 10678
Dataset Kind Image data
Dataset Size 470.3 MB
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