Summary of 27-Iwasaki-iPSCellDyn

SSBD:database
SSBD:database URL
Title
-
Description
-
Relase date
2017-10-03
Updated date
2018-11-15
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
13 ( Image datasets: 13, Quantitative data datasets: 0 )
Size of Datasets
327.0 MB ( Image datasets: 327.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. musculus
Strain(s)
iPS
Gene symbol(s)
Nrl
Protein tag(s)
GFP

Datatype
cell/tissue structure
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell
Biological Imaging Method
-
XYZ Scale
XY: 3.24 micrometer/pixel, Z: NA, XY: 0.847 micrometer/pixel, Z: NA, XY: 0.424 micrometer/pixel, Z: NA, XY: 0.342 nanometer/pixel, Z: NA, XY: 1.13 nanometer/pixel, Z: NA, XY: 4.26 nanometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
JEOL JEM 1400PLUS,  a motorized inverted system microscope (IX71, Olympus)
Detector model
CCD camera (2048 x 2048) attached to the JEOL JEM 1400PLUS, charge-coupled device camera (DP72, Olympus), and digital imaging software (DP2-BSW, Olympus)
Objective model
Olympus UPlanFL 10 × N.A. 0.30 ph1, Olympus LUCPlanFL 20 × 0.45 ph1, NA, Olympus UPlanFL 4 × 0.13 phL
Filter set
-

Related paper(s)

Yuko Iwasaki, Sunao Sugita, Michiko Mandai, Shigenobu Yonemura, Akishi Onishi, Shin-Ichiro Ito, Manabu Mochizuki, Kyoko Ohno-Matsui, Masayo Takahashi (2016) Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool., PloS one, Volume 11, Number 7, pp. e0158282

Published in 2016 (Electronic publication in July 6, 2016, midnight )

(Abstract) PURPOSE: To establish a novel protocol for differentiation of retinal pigment epithelium (RPE) with high purity from mouse induced pluripotent stem cells (iPSC). METHODS: Retinal progenitor cells were differentiated from mouse iPSC, and RPE differentiation was then enhanced by activation of the Wnt signaling pathway, inhibition of the fibroblast growth factor signaling pathway, and inhibition of the Rho-associated, coiled-coil containing protein kinase signaling pathway. Expanded pigmented cells were purified by plate adhesion after Accutase(R) treatment. Enriched cells were cultured until they developed a cobblestone appearance with cuboidal shape. The characteristics of iPS-RPE were confirmed by gene expression, immunocytochemistry, and electron microscopy. Functions and immunologic features of the iPS-RPE were also evaluated. RESULTS: We obtained iPS-RPE at high purity (approximately 98%). The iPS-RPE showed apical-basal polarity and cellular structure characteristic of RPE. Expression levels of several RPE markers were lower than those of freshly isolated mouse RPE but comparable to those of primary cultured RPE. The iPS-RPE could form tight junctions, phagocytose photoreceptor outer segments, express immune antigens, and suppress lymphocyte proliferation. CONCLUSION: We successfully developed a differentiation/purification protocol to obtain mouse iPS-RPE. The mouse iPS-RPE can serve as an attractive tool for functional and morphological studies of RPE.
(MeSH Terms)

Contact
Masayo Takahashi , RIKEN , Center for Developmental Biology , Laboratory for Retinal Regeneration
Contributors
Yuko Iwasaki, Sunao Sugita, Michiko Mandai, Shigenobu Yonemura, Akishi Onishi, Shin-ichiro Ito, Manabu Mochizuki Kyoko Ohno-Matsui, Masayo Takahashi


Dataset List of 27-Iwasaki-iPSCellDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 887
Datast ID Fig1b_DD7
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 888
Datast ID Fig1d_DD7
Dataset Kind Image data
Dataset Size 4.0 MB
4D view
SSBD:OMERO
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# 889
Datast ID Fig1e_DD7
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 890
Datast ID Fig1f_DD11
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 891
Datast ID Fig1g_DD11
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 892
Datast ID Fig1i_DD16
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 893
Datast ID Fig1c-2_DD26
Dataset Kind Image data
Dataset Size 4.0 MB
4D view
SSBD:OMERO
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# 894
Datast ID Fig1c-1_DD26
Dataset Kind Image data
Dataset Size 4.0 MB
4D view
SSBD:OMERO
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# 895
Datast ID Fig1k_DD29
Dataset Kind Image data
Dataset Size 9.2 MB
4D view
SSBD:OMERO
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# 896
Datast ID Fig2b_DD29
Dataset Kind Image data
Dataset Size 64.4 MB
4D view
SSBD:OMERO
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# 897
Datast ID Fig2c_DD29
Dataset Kind Image data
Dataset Size 65.4 MB
4D view
SSBD:OMERO
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# 898
Datast ID Fig2d_DD29
Dataset Kind Image data
Dataset Size 65.0 MB
4D view
SSBD:OMERO
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# 899
Datast ID Fig2e_DD29
Dataset Kind Image data
Dataset Size 64.8 MB
4D view
SSBD:OMERO
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