Summary of 239-Miyamoto-MitochondrialDyn

SSBD:database
SSBD:database URL
Title
Living-cell imaging of HeLa cells transfected with Tom20-CR.
Description
-
Relase date
2022-11-23
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
160.5 MB ( Image datasets: 160.5 MB, Quantitative data datasets: 0 bytes )

Organism(s)
Homo sapiens
Cell lines(s)
HeLa cell
Reporter(s)
Tom20-CR

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrial chromosome
Biological Imaging Method
fluorescence microscopy
X scale
0.1647955 micrometer/pixel
Y scale
0.1647955 micrometer/pixel
Z scale
1 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Takafumi Miyamoto, Song-Iee Han, Hitoshi Shimano (2021) Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)., STAR protocols, Volume 2, Number 3, pp. 100721

Published in 2021 Sep 17 (Electronic publication in Aug. 5, 2021, midnight )

(Abstract) Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.
(MeSH Terms)

Contact
Takafumi Miyamoto , University of Tsukuba , Faculty of Medicine , Department of Internal Medicine (Endocrinology and Metabolism)
Contributors


Dataset List of 239-Miyamoto-MitochondrialDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
Download BDML
Download Images
# 8781
Dataset Kind Image data
Dataset Size 160.5 MB
4D view
SSBD:OMERO
Download BDML
Download Image data