Summary of 207-Kuroda-FinMorphology

SSBD:database URL
3D FIB-SEM images of a cross section of fin mesenchymal cells and actinotrichia in zebrafish.
Relase date
Updated date
Image data based on Experiment
Number of Datasets
1 ( Image datasets: 1, Quantitative data datasets: 0 )
Size of Datasets
248.1 MB ( Image datasets: 248.1 MB, Quantitative data datasets: 0 bytes )

Danio rerio

Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
basement membrane
Biological Imaging Method
scanning electron microscopy
X scale
8.26 nanometer/pixel
Y scale
10.5 nanometer/pixel
Z scale
15 nanometer/slice
T scale

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Related paper(s)

Junpei Kuroda, Takeshi Itabashi, Atsuko H Iwane, Toshihiro Aramaki, Shigeru Kondo (2020) The Physical Role of Mesenchymal Cells Driven by the Actin Cytoskeleton Is Essential for the Orientation of Collagen Fibrils in Zebrafish Fins., Frontiers in cell and developmental biology, Volume 8, pp. 580520

Published in 2020 (Electronic publication in Oct. 14, 2020, midnight )

(Abstract) Fibrous collagen imparts physical strength and flexibility to tissues by forming huge complexes. The density and orientation of collagen fibers must be correctly specified for the optimal physical property of the collagen complex. However, little is known about its underlying cellular mechanisms. Actinotrichia are collagen fibers aligned at the fin-tip of bony fish and are easily visible under the microscope due to their thick, linear structure. We used the actinotrichia as a model system to investigate how cells manipulate collagen fibers. The 3D image obtained by focused ion beam scanning electron microscopy (FIB-SEM) showed that the pseudopodia of mesenchymal cells encircle the multiple actinotrichia. We then co-incubated the mesenchymal cells and actinotrichia in vitro, and time-lapse analysis revealed how cells use pseudopods to align collagen fiber orientation. This in vitro behavior is dependent on actin polymerization in mesenchymal cells. Inhibition of actin polymerization in mesenchymal cells results in mis-orientation of actinotrichia in the fin. These results reveal how mesenchymal cells are involved in fin formation and have important implications for the physical interaction between cells and collagen fibers.

Shigeru Kondo , Osaka University , Graduate School of Frontier Bioscience

Dataset List of 207-Kuroda-FinMorphology

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# 7534
Dataset Kind Image data
Dataset Size 248.1 MB
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