Summary of 202-Miyagawa-ChemotaxisDyn

SSBD:database
SSBD:database URL
Title
Time-lapse images of chemotaxis trajectories of gip1Δ cells harbouring vecotr, Gip1(wild-type), Gip1(D208A), or Gip1(ΔC-tail)
Description
-
Relase date
2022-03-31
Updated date
-
License
CC-BY
Kind
Image data based on Experiment
Number of Datasets
4 ( Image datasets: 4, Quantitative data datasets: 0 )
Size of Datasets
3.9 GB ( Image datasets: 3.9 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Dictyostelium discoideum
Strain(s)
gip1Δ cell
Gene symbol(s)
gip1

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
chemotaxis
Cellular Component (CC)
-
Biological Imaging Method
time lapse microscopy
X scale
2.4859 micrometer/pixel
Y scale
2.4859 micrometer/pixel
Z scale
-
T scale
10 second per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Takero Miyagawa, Hiroyasu Koteishi, Yoichiro Kamimura, Yukihiro Miyanaga, Kohei Takeshita, Atsushi Nakagawa, Masahiro Ueda (2018) Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis., Nature communications, Volume 9, Number 1, pp. 4635

Published in 2018 Nov 6 (Electronic publication in Nov. 6, 2018, midnight )

(Abstract) G protein interacting protein 1 (Gip1) binds and sequesters heterotrimeric G proteins in the cytosolic pool, thus regulating G protein-coupled receptor (GPCR) signalling for eukaryotic chemotaxis. Here, we report the underlying structural basis of Gip1 function. The crystal structure reveals that the region of Gip1 that binds to the G protein has a cylinder-like fold with a central hydrophobic cavity composed of six alpha-helices. Mutagenesis and biochemical analyses indicate that the hydrophobic cavity and the hydrogen bond network at the entrance of the cavity are essential for complex formation with the geranylgeranyl modification on the Ggamma subunit. Mutations of the cavity impair G protein sequestration and translocation to the membrane from the cytosol upon receptor stimulation, leading to defects in chemotaxis at higher chemoattractant concentrations. These results demonstrate that the Gip1-dependent regulation of G protein shuttling ensures wide-range gradient sensing in eukaryotic chemotaxis.
(MeSH Terms)

Contact
Masahiro Ueda , Osaka University , Graduate School of Frontier Biosciences , Laboratory for Cell Signaling Dynamics
Contributors


Dataset List of 202-Miyagawa-ChemotaxisDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 7574
Dataset Kind Image data
Dataset Size 1004.1 MB
4D view
SSBD:OMERO
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# 7575
Dataset Kind Image data
Dataset Size 1004.1 MB
4D view
SSBD:OMERO
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# 7576
Dataset Kind Image data
Dataset Size 1004.2 MB
4D view
SSBD:OMERO
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# 7577
Dataset Kind Image data
Dataset Size 1004.1 MB
4D view
SSBD:OMERO
Download BDML
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