Summary of 165-Kinjo-ERKactivity

SSBD:database
SSBD:database URL
Title
Live imaging data of ERK activity in the MDCK cyte or mouse ear epidermis before or after 2p excitation.
Description
-
Relase date
2021-09-30
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
20 ( Image datasets: 20, Quantitative data datasets: 0 )
Size of Datasets
1.1 GB ( Image datasets: 1.1 GB, Quantitative data datasets: 0 bytes )

Organism(s)
Mus musculus, Canis lupus familiaris
Cell lines(s)
MDCK cell
Protein name(s)
2paRAF, ERK

Datatype
-
Molecular Function (MF)
extracellular signal-regulated kinase activity
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
time lapse microscopy, FRET
X scale
0.265 micrometer/pixel, 0.397 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.662 micrometer/pixel, 0.198 micrometer/pixel
Y scale
0.265 micrometer/pixel, 0.397 micrometer/pixel, 0.795 micrometer/pixel, 0.53 micrometer/pixel, 0.662 micrometer/pixel, 0.198 micrometer/pixel
Z scale
2 micrometer/slice, 1 micrometer/slice
T scale
0.08 sec per time interval, NA, 1.03 sec per time interval, 0.12 sec per time interval, 322.12 sec per time interval, 0.06 sec per time interval, 120.12 sec per time interval, 366.47 sec per time interval, 120.13 sec per time interval, 333.24 sec per time interval, 61.61 sec per time interval, 180.12 sec per time interval, 0.11 sec per time interval, 1 sec per time interval, 63.40 sec per time interval, 180.13 sec per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Tomoaki Kinjo, Kenta Terai, Shoichiro Horita, Norimichi Nomura, Kenta Sumiyama, Kaori Togashi, So Iwata, Michiyuki Matsuda (2019) FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics., Nature methods, Volume 16, Number 10, pp. 1029-1036

Published in 2019 Oct (Electronic publication in Sept. 9, 2019, midnight )

(Abstract) Optical dimerizers have been developed to untangle signaling pathways, but they are of limited use in vivo, partly due to their inefficient activation under two-photon (2P) excitation. To overcome this problem, we developed Forster resonance energy transfer (FRET)-assisted photoactivation, or FRAPA. On 2P excitation, mTagBFP2 efficiently absorbs and transfers the energy to the chromophore of CRY2. Based on structure-guided engineering, a chimeric protein with 40% FRET efficiency was developed and named 2P-activatable CRY2, or 2paCRY2. 2paCRY2 was employed to develop a RAF1 activation system named 2paRAF. In three-dimensionally cultured cells expressing 2paRAF, extracellular signal-regulated kinase (ERK) was efficiently activated by 2P excitation at single-cell resolution. Photoactivation of ERK was also accomplished in the epidermal cells of 2paRAF-expressing mice. We further developed an mTFP1-fused LOV domain that exhibits efficient response to 2P excitation. Collectively, FRAPA will pave the way to single-cell optical control of signaling pathways in vivo.
(MeSH Terms)

Contact
Michiyuki Matsuda, Kenta Terai , Graduate School of Biostudies, Kyoto University, Graduate School of Biostudies, Kyoto University , Research Center for Dynamic Living Systems, Research Center for Dynamic Living Systems , Laboratory of Bioimaging and Cell Signaling, Laboratory of Bioimaging and Cell Signaling
Contributors


Dataset List of 165-Kinjo-ERKactivity

#
Dataset ID
Kind
Size
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# 5039
Dataset Kind Image data
Dataset Size 207.0 MB
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# 5040
Datast ID Fig4b_postHgL_1p
Dataset Kind Image data
Dataset Size 148.0 MB
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SSBD:OMERO
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# 5041
Dataset Kind Image data
Dataset Size 89.1 MB
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SSBD:OMERO
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# 5042
Dataset Kind Image data
Dataset Size 2.0 MB
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SSBD:OMERO
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# 5043
Dataset Kind Image data
Dataset Size 208.3 MB
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SSBD:OMERO
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# 5044
Datast ID Fig4g_postHgL_1p
Dataset Kind Image data
Dataset Size 208.3 MB
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SSBD:OMERO
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# 5045
Dataset Kind Image data
Dataset Size 104.4 MB
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SSBD:OMERO
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# 5046
Dataset Kind Image data
Dataset Size 2.4 MB
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SSBD:OMERO
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# 5047
Dataset Kind Image data
Dataset Size 22.1 MB
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SSBD:OMERO
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# 5048
Dataset Kind Image data
Dataset Size 22.1 MB
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SSBD:OMERO
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# 5049
Dataset Kind Image data
Dataset Size 2.6 MB
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SSBD:OMERO
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# 5050
Datast ID Fig5a_mScarlet
Dataset Kind Image data
Dataset Size 3.2 MB
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SSBD:OMERO
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# 5051
Dataset Kind Image data
Dataset Size 3.2 MB
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SSBD:OMERO
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# 5052
Dataset Kind Image data
Dataset Size 21.1 MB
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SSBD:OMERO
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# 5053
Dataset Kind Image data
Dataset Size 21.1 MB
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SSBD:OMERO
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# 5054
Dataset Kind Image data
Dataset Size 3.7 MB
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SSBD:OMERO
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# 5055
Dataset Kind Image data
Dataset Size 3.2 MB
4D view
SSBD:OMERO
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# 5056
Dataset Kind Image data
Dataset Size 21.1 MB
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SSBD:OMERO
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# 5057
Dataset Kind Image data
Dataset Size 21.1 MB
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SSBD:OMERO
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# 5058
Dataset Kind Image data
Dataset Size 2.7 MB
4D view
SSBD:OMERO
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