Summary of 111-Kawaguchi-NPCellsDyn

SSBD:database
SSBD:database URL
Title
Time-lapse and fluorescent images of collectice dynamics of neural progenitor cells (NPCs)
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Quantitative data, Image data based on Experiment
Number of Datasets
32 ( Image datasets: 30, Quantitative data datasets: 2 )
Size of Datasets
15.7 GB ( Image datasets: 15.7 GB, Quantitative data datasets: 12.3 MB )

Organism(s)
M. musculus
Protein name(s)
H2B
Protein tag(s)
mCherry

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
interkinetic nuclear migration
Cellular Component (CC)
nucleus
Biological Imaging Method
-
XYZ Scale
XY: 0.33 micrometer/pixel, Z: a.u., XY: 0.65 micrometer/pixel, Z: a.u., XY: 0.92 micrometer/pixel, Z: a.u., XY: 0.94 micrometer/pixel, Z: a.u.
T scale
NA, 5 minutes for each time interval

Image Acquisition
Experiment type
Immunofluorescence, Other, TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
NA, Fluorescence, Phase
Microscope model
Leica AF7000, Nikon Eclipse Ti-E, Leica AF6000
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Kyogo Kawaguchi, Ryoichiro Kageyama, Masaki Sano (2017) Topological defects control collective dynamics in neural progenitor cell cultures., Nature, Volume 545, Number 7654, pp. 327-331

Published in 2017 Apr 12 (Electronic publication in April 12, 2017, midnight )

(Abstract) Cultured stem cells have become a standard platform not only for regenerative medicine and developmental biology but also for biophysical studies. Yet, the characterization of cultured stem cells at the level of morphology and of the macroscopic patterns resulting from cell-to-cell interactions remains largely qualitative. Here we report on the collective dynamics of cultured murine neural progenitor cells (NPCs), which are multipotent stem cells that give rise to cells in the central nervous system. At low densities, NPCs moved randomly in an amoeba-like fashion. However, NPCs at high density elongated and aligned their shapes with one another, gliding at relatively high velocities. Although the direction of motion of individual cells reversed stochastically along the axes of alignment, the cells were capable of forming an aligned pattern up to length scales similar to that of the migratory stream observed in the adult brain. The two-dimensional order of alignment within the culture showed a liquid-crystalline pattern containing interspersed topological defects with winding numbers of +1/2 and -1/2 (half-integer due to the nematic feature that arises from the head-tail symmetry of cell-to-cell interaction). We identified rapid cell accumulation at +1/2 defects and the formation of three-dimensional mounds. Imaging at the single-cell level around the defects allowed us to quantify the velocity field and the evolving cell density; cells not only concentrate at +1/2 defects, but also escape from -1/2 defects. We propose a generic mechanism for the instability in cell density around the defects that arises from the interplay between the anisotropic friction and the active force field.

Contact
Kyogo Kawaguchi , RIKEN , Center for Biosystems Dynamics Research , Nonequilibrium physics of living matter RIKEN Hakubi Research Team
Contributors
Kyogo Kawaguchi, Ryoichiro Kageyama, Masaki Sano


Dataset List of 111-Kawaguchi-NPCellsDyn

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4460
Datast ID fig1a_FullRaw_mCh
Dataset Kind Image data
Dataset Size 795.4 MB
4D view
SSBD:OMERO
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# 4461
Datast ID fig1a_FullRaw_Ph
Dataset Kind Image data
Dataset Size 795.4 MB
4D view
SSBD:OMERO
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# 4462
Datast ID fig4a_bottom
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4463
Datast ID fig4a_top
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4464
Datast ID fig4b_merge
Dataset Kind Image data
Dataset Size 150.1 MB
4D view
SSBD:OMERO
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# 4465
Datast ID fig4g_left
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4466
Datast ID fig4g_right
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4467
Datast ID extended_Fig7
Dataset Kind Image data
Dataset Size 3.1 MB
4D view
SSBD:OMERO
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# 4468
Datast ID extended_Fig8
Dataset Kind Image data
Dataset Size 11.7 MB
4D view
SSBD:OMERO
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# 4469
Dataset Kind Image data
Dataset Size 8.0 MB
4D view
SSBD:OMERO
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# 4470
Dataset Kind Image data
Dataset Size 4.0 MB
4D view
SSBD:OMERO
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# 4471
Dataset Kind Image data
Dataset Size 2.0 MB
4D view
SSBD:OMERO
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# 4472
Dataset Kind Image data
Dataset Size 4.0 MB
4D view
SSBD:OMERO
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# 4473
Dataset Kind Image data
Dataset Size 800.6 KB
4D view
SSBD:OMERO
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# 4474
Dataset Kind Image data
Dataset Size 800.6 KB
4D view
SSBD:OMERO
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# 4475
Datast ID SuppMovie_SV1_left
Dataset Kind Image data
Dataset Size 620.7 MB
4D view
SSBD:OMERO
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# 4476
Dataset Kind Image data
Dataset Size 620.7 MB
4D view
SSBD:OMERO
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# 4477
Datast ID SuppMovie_SV2_left
Dataset Kind Image data
Dataset Size 3.5 GB
4D view
SSBD:OMERO
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# 4478
Dataset Kind Image data
Dataset Size 3.5 GB
4D view
SSBD:OMERO
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# 4479
Datast ID SuppMovie_SV3
Dataset Kind Image data
Dataset Size 708.0 MB
4D view
SSBD:OMERO
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# 4480
Datast ID SuppMovie_SV4_left
Dataset Kind Image data
Dataset Size 542.5 MB
4D view
SSBD:OMERO
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# 4481
Dataset Kind Image data
Dataset Size 542.7 MB
4D view
SSBD:OMERO
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# 4482
Dataset Kind Image data
Dataset Size 339.5 MB
4D view
SSBD:OMERO
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# 4483
Datast ID SuppMovie_SV5_top
Dataset Kind Image data
Dataset Size 339.5 MB
4D view
SSBD:OMERO
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# 4484
Dataset Kind Image data
Dataset Size 795.4 MB
4D view
SSBD:OMERO
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# 4485
Datast ID SuppMovie_SV6_red
Dataset Kind Image data
Dataset Size 795.4 MB
4D view
SSBD:OMERO
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# 4486
Datast ID SuppMovie_SV7_left
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4487
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4488
Datast ID SuppMovie_SV8_left
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4489
Dataset Kind Image data
Dataset Size 225.1 MB
4D view
SSBD:OMERO
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# 4490
Datast ID fig2a_track1
Dataset Kind Quantitative data
Dataset Size 4.2 MB
4D view
SSBD:OMERO
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# 4491
Datast ID fig2a_track2
Dataset Kind Quantitative data
Dataset Size 8.1 MB
4D view
SSBD:OMERO
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