Summary of 108-Sugita-RPEiPSCell

SSBD:database
SSBD:database URL
Title
Immunofluorescence images of 46a IPS-RPE cells incubaed with the serum from the TLHM6 monkey in different stage
Description
-
Relase date
2019-11-20
Updated date
-
License
CC BY
Kind
Image data based on Experiment
Number of Datasets
9 ( Image datasets: 9, Quantitative data datasets: 0 )
Size of Datasets
6.0 MB ( Image datasets: 6.0 MB, Quantitative data datasets: 0 bytes )

Organism(s)
M. fascicularis
Strain(s)
iPS

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
retinal pigment epithelium development
Cellular Component (CC)
cell
Biological Imaging Method
-
XYZ Scale
XY: 1.2503 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
ZEISS LSM700
Detector model
-
Objective model
-
Filter set
-

Related paper(s)

Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi (2017) Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models., Stem cell reports, Volume 9, Number 5, pp. 1501-1515

Published in 2017 Nov 14 (Electronic publication in Nov. 5, 2017, midnight )

(Abstract) Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models. We detected alloantibodies in the serum from recipient monkeys that had immune attacks in the retina in an immunofluorescent assay using the transplanted iPSC-RPE cells as the antigen. In addition to T cell and antigen-presenting cell immunity, peripheral blood cells and lymph nodes in animal models with allogeneic iPSC-RPE cells also had activated B cells, which were probably secreting alloantibodies. Using serum and transplanted cells, alloreactive antibody can be detected for the diagnosis of immune rejection after transplantation.
(MeSH Terms)

Contact
Sunao Sugita , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Retinal Regeneration
Contributors
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi


Dataset List of 108-Sugita-RPEiPSCell

#
Dataset ID
Kind
Size
4D View
SSBD:OMERO
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# 4426
Dataset Kind Image data
Dataset Size 927.6 KB
4D view
SSBD:OMERO
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# 4427
Dataset Kind Image data
Dataset Size 666.3 KB
4D view
SSBD:OMERO
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# 4428
Dataset Kind Image data
Dataset Size 651.2 KB
4D view
SSBD:OMERO
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# 4429
Dataset Kind Image data
Dataset Size 668.6 KB
4D view
SSBD:OMERO
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# 4430
Dataset Kind Image data
Dataset Size 650.2 KB
4D view
SSBD:OMERO
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# 4431
Dataset Kind Image data
Dataset Size 648.6 KB
4D view
SSBD:OMERO
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# 4432
Dataset Kind Image data
Dataset Size 687.2 KB
4D view
SSBD:OMERO
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# 4433
Dataset Kind Image data
Dataset Size 637.2 KB
4D view
SSBD:OMERO
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# 4434
Datast ID fig7_MouseIgG
Dataset Kind Image data
Dataset Size 634.4 KB
4D view
SSBD:OMERO
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Download Image data