Detail of Fig2-Merge



Project
Title
Time-lapse microscopy images of an aTRLF cell colony (tTS-2A-GFP and Delta-2A-mCherry)
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
File Formats
Data size
98.7 MB

Organism
C. griseus ( NCBI:txid10029 )
Strain(s)
CHO
Cell Line
-
Protein names
tTS, Delta
Protein tags
GFP, mCherry

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 1.0 micrometer/pixel, Z: NA
T scale
1 hour for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Matsuda et al. (2015) Nat. Commun., 6, 6195
Related paper(s)

Mitsuhiro Matsuda, Makito Koga, Knut Woltjen, Eisuke Nishida, Miki Ebisuya (2015) Synthetic lateral inhibition governs cell-type bifurcation with robust ratios., Nature communications, Volume 6, pp. 6195

Published in 2015 Feb 5 (Electronic publication in Feb. 5, 2015, midnight )

(Abstract) Cell-type diversity in multicellular organisms is created through a series of binary cell fate decisions. Lateral inhibition controlled by Delta-Notch signalling is the core mechanism for the choice of alternative cell types by homogeneous neighbouring cells. Here, we show that cells engineered with a Delta-Notch-dependent lateral inhibition circuit spontaneously bifurcate into Delta-positive and Notch-active cell populations. The synthetic lateral inhibition circuit comprises transcriptional repression of Delta and intracellular feedback of Lunatic fringe (Lfng). The Lfng-feedback subcircuit, even alone, causes the autonomous cell-type bifurcation. Furthermore, the ratio of two cell populations bifurcated by lateral inhibition is reproducible and robust against perturbation. The cell-type ratio is adjustable by the architecture of the lateral inhibition circuit as well as the degree of cell-cell attachment. Thus, the minimum lateral inhibition mechanism between adjacent cells not only serves as a binary cell-type switch of individual cells but also governs the cell-type ratio at the cell-population level.
(MeSH Terms)

Contact
Miki Ebisuya , RIKEN , Quantitative Biology Center , Laboratory for Reconstitutive Developmental Biology
Contributors
Mitsuhiro Matsuda, Makito Koga, Knut Woltjen, Eisuke Nishida, Miki Ebisuya

OMERO Dataset
OMERO Project
Source