Detail of MovieS3-EG-Chd



Project
Title
Confocal microscopy images of FRAP assay of mEGFP-tagged Chordin in X. laevis
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
File Formats
Data size
290.3 MB

Organism
X. laevis ( NCBI:txid8355 )
Strain(s)
-
Cell Line
-
Protein names
Chordin
Protein tags
mEGFP

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
NA (protein diffusion)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.267 micrometer/pixel, Z: 0 micrometer/frame
T scale
0.97 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM710
Detector model
-
Objective model
Carl Zeiss Plan-Apochromat 20x/0.8
Filter set
-

Summary of Methods
See details in Inomata et al. (2013) Cell, 153(6): 1296-1311.
Related paper(s)

Hidehiko Inomata, Tatsuo Shibata, Tomoko Haraguchi, Yoshiki Sasai (2013) Scaling of dorsal-ventral patterning by embryo size-dependent degradation of Spemann's organizer signals., Cell, Volume 153, Number 6, pp. 1296-311

Published in 2013 Jun 6

(Abstract) Spemann's organizer plays a key role in dorsal-ventral (DV) patterning in the amphibian embryo by secreting diffusible proteins such as Chordin, an antagonist to ventralizing bone morphogenetic proteins (BMPs). The DV patterning is so robust that an amphibian embryo with its ventral half surgically removed can develop into a smaller but proportionally patterned larva. Here, we show that this robust patterning depends on facilitated Chordin degradation and requires the expression of the Chordin-proteinase inhibitor Sizzled on the opposite side. Sizzled, which is stable and diffuses widely along the DV axis, stabilizes Chordin and expands its distribution in the ventral direction. This expanded Chordin distribution, in turn, limits BMP-dependent Sizzled production, forming an axis-wide feedback loop for shaping Chordin's activity. Using bisection assays, we demonstrate that Chordin degradation is dynamically controlled by embryo-size-coupled Sizzled accumulation. We propose a scaling model that enables the DV pattern to adjust proportionally to embryonic axis size.
(MeSH Terms)

Contact
Hidehiko Inomata , RIKEN , Center for Developmental Biology , Laboratory for Physical Biology
Contributors
Hidehiko Inomata, Tatsuo Shibata, Tomoko Haraguchi, Yoshiki Sasai

OMERO Dataset
OMERO Project
Source