Detail of Fig5c_Spectroscopic_OR-PAM



Project
Title
Spectroscopic optical-resolution photoacoustic microscopy (OR-PAM) image of the planar-cultured cells.
Description
Spectroscopic optical-resolution photoacoustic microscopy (OR-PAM) image of the planar-cultured cells.
Release, Updated
2022-12-13
License
CC BY
Kind
Image data
File Formats
.tif
Data size
276.9 KB

Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
-
Cell Line
HeLa cell ( CLO_0003684 )

Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
photoacoustic microscopy
X scale
2.0 micrometer/pixel
Y scale
2.0 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Hirasawa T, et. al. (2022) Photoacoustics, May 7;26:100364.
Related paper(s)

Takeshi Hirasawa, Kazuyoshi Tachi, Manami Miyashita, Shinpei Okawa, Toshihiro Kushibiki, Miya Ishihara (2022) Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay., Photoacoustics, Volume 26, pp. 100364

Published in 2022 Jun (Electronic publication in May 7, 2022, midnight )

(Abstract) In most multispectral optical-resolution photoacoustic microscopy (OR-PAM), spatial scanning is repeated for each excitation wavelength, which decreases throughput and causes motion artifacts during spectral processing. This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans. The technique involves irradiating an imaging target with two broadband optical pulses with and without wavelength-dependent time delays. The excitation wavelength of the sample is then calculated by measuring the time delay between the photoacoustic signals generated by the two optical pulses. This technique is validated by measuring the excitation wavelengths of dyes in tubes. Furthermore, we demonstrate the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes. Although the tradeoff between excitation efficiency and excitation bandwidth must be adjusted based on the application, combining the proposed technique with fast spatial scanning methods can significantly contribute to recent OR-PAM applications, such as monitoring quick biological events and microscale tracking of moving materials.

Contact
Miya Ishihara, Takeshi Hirasawa , National Defense Medical College, National Defense Medical College , Department of Medical Engineering, Department of Medical Engineering
Contributors

OMERO Dataset
OMERO Project
Source