Detail of Figure1B_haploid_thymidine

Live images of SiR-tubulin-stained haploid HAP1 cells treated with thymidine.
Live images of SiR-tubulin-stained haploid HAP1 cells treated with thymidine.
Release, Updated
Image data
File Formats
uncompressed TIFF
Data size
40.5 GB

Homo sapiens ( NCBITaxon:9606 )
Cell Line
HAP1 cell ( NA )

Molecular Function (MF)
Biological Process (BP)
mitotic cell cycle ( GO:0000278 )
Cellular Component (CC)
centrosome ( GO:0005813 ) chromosome ( GO:0005694 )
Biological Imaging Method
Ti-2 microscope with a × 40 0.95 NA Plan-Apochromatic objective lens (Nikon)
and Zyla4.2 sCMOS camera (Andor)
X scale
0.1666695 micrometer/pixel
Y scale
0.1666695 micrometer/pixel
Z scale
3 micrometer/slice
T scale
5 minutes per time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Yoshizawa K, et. al. (2020) Front Cell Dev Biol, Jul 30;8:721.
Related paper(s)

Koya Yoshizawa, Kan Yaguchi, Ryota Uehara (2020) Uncoupling of DNA Replication and Centrosome Duplication Cycles Is a Primary Cause of Haploid Instability in Mammalian Somatic Cells., Frontiers in cell and developmental biology, Volume 8, pp. 721

Published in 2020 (Electronic publication in July 30, 2020, midnight )

(Abstract) Mammalian haploid somatic cells are unstable and prone to diploidize, but the cause of haploid instability remains largely unknown. Previously, we found that mammalian haploid somatic cells suffer chronic centrosome loss stemming from the uncoupling of DNA replication and centrosome duplication cycles. However, the lack of methodology to restore the coupling between DNA replication and centrosome duplication has precluded us from investigating the potential contribution of the haploidy-linked centrosome loss to haploid instability. In this study, we developed an experimental method that allows the re-coupling of DNA and centrosome cycles through the chronic extension of the G1/S phase without compromising cell proliferation using thymidine treatment/release cycles. Chronic extension of G1/S restored normal mitotic centrosome number and mitotic control, substantially improving the stability of the haploid state in HAP1 cells. Stabilization of the haploid state was compromised when cdk2 was inhibited during the extended G1/S, or when early G1 was chronically extended instead of G1/S, showing that the coupling of DNA and centrosome cycles rather than a general extension of the cell cycle is required for haploid stability. Our data indicate the chronic centriole loss arising from the uncoupling of centrosome and DNA cycles as a direct cause of genome instability in haploid somatic cells, and also demonstrate the feasibility of modulation of haploid stability through artificial coordination between DNA and centrosome cycles in mammalian somatic cells.

Ryota Uehara , Hokkaido University , Faculty of Advanced Life Science

OMERO Dataset
OMERO Project