Detail of Fig4B_ASW_pre



Project
Title
Time-sereis images of chemotaxis behavior of N. humilis treated without platytocin.
Description
Time-sereis images of chemotaxis behavior of N. humilis treated without platytocin.
Release, Updated
2022-11-23
License
CC BY
Kind
Image data
File Formats
.png
Data size
629.5 MB

Organism
Notocomplana humilis ( NCBI:txid1641631 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
chemotaxis ( GO:0006935 )
Cellular Component (CC)
Biological Imaging Method
HDR-CX680/W video recorder
X scale
0.159 millimeter per pixel
Y scale
0.159 millimeter per pixel
Z scale
-
T scale
5 seconds per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Kobayashi A, et. al. (2022) Sci Adv, Mar 4;8(9):eabk0331.
Related paper(s)

Aoshi Kobayashi, Mayuko Hamada, Masa-Aki Yoshida, Yasuhisa Kobayashi, Naoaki Tsutsui, Toshio Sekiguchi, Yuta Matsukawa, Sho Maejima, Joseph J Gingell, Shoko Sekiguchi, Ayumu Hamamoto, Debbie L Hay, John F Morris, Tatsuya Sakamoto, Hirotaka Sakamoto (2022) Vasopressin-oxytocin-type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians., Science advances, Volume 8, Number 9, pp. eabk0331

Published in 2022 Mar 4 (Electronic publication in March 4, 2022, midnight )

(Abstract) Vasopressin/oxytocin (VP/OT)-related peptides are essential for mammalian antidiuresis, sociosexual behavior, and reproduction. However, the evolutionary origin of this peptide system is still uncertain. Here, we identify orthologous genes to those for VP/OT in Platyhelminthes, intertidal planarians that have a simple bilaterian body structure but lack a coelom and body-fluid circulatory system. We report a comprehensive characterization of the neuropeptide derived from this VP/OT-type gene, identifying its functional receptor, and name it the "platytocin" system. Our experiments with these euryhaline planarians, living where environmental salinities fluctuate due to evaporation and rainfall, suggest that platytocin functions as an "antidiuretic hormone" and also organizes diverse actions including reproduction and chemosensory-associated behavior. We propose that bilaterians acquired physiological adaptations to amphibious lives by such regulation of the body fluids. This neuropeptide-secreting system clearly became indispensable for life even without the development of a vascular circulatory system or relevant synapses.

Contact
Hirotaka Sakamoto , Okayama University , Graduate School of Natural Science and Tech- nology , Ushimado Marine Institute (UMI)
Contributors
Aoshi Kobayashi

OMERO Dataset
OMERO Project
Source