Detail of Whole_brain_POGZWT-Q1038R_20170830_10015



Project
Title
3D whole-brain image showing social-interaction-induced activation of Arc-dVenus fluorescence in POGZWT/Q1038R mice.
Description
3D whole-brain image showing social-interaction-induced activation of Arc-dVenus fluorescence in POGZWT/Q1038R mice.
Release, Updated
2022-11-23
License
CC BY-NC-SA
Kind
Image data
File Formats
.tif
Data size
4.7 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Arc
Protein tags
dVenus

Datatype
-
Molecular Function (MF)
Biological Process (BP)
brain development ( GO:0007420 )
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
whole-brain imaging
X scale
1.0 micrometer/pixel
Y scale
1.0 micrometer/pixel
Z scale
5 micrometer/pixel
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Matsumura K, et. al. (2020) Nat Commun, Feb 26;11(1):859.
Related paper(s)

Kensuke Matsumura, Kaoru Seiriki, Shota Okada, Masashi Nagase, Shinya Ayabe, Ikuko Yamada, Tamio Furuse, Hirotoshi Shibuya, Yuka Yasuda, Hidenaga Yamamori, Michiko Fujimoto, Kazuki Nagayasu, Kana Yamamoto, Kohei Kitagawa, Hiroki Miura, Nanaka Gotoda-Nishimura, Hisato Igarashi, Misuzu Hayashida, Masayuki Baba, Momoka Kondo, Shigeru Hasebe, Kosei Ueshima, Atsushi Kasai, Yukio Ago, Atsuko Hayata-Takano, Norihito Shintani, Tokuichi Iguchi, Makoto Sato, Shun Yamaguchi, Masaru Tamura, Shigeharu Wakana, Atsushi Yoshiki, Ayako M. Watabe, Hideyuki Okano, Kazuhiro Takuma, Ryota Hashimoto, Hitoshi Hashimoto, Takanobu Nakazawa (2020) Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes., Nature Communications, Volume 11

Published in 2020 (Electronic publication in Feb. 26, 2020, midnight )

(Abstract) Pogo transposable element derived with ZNF domain (POGZ) has been identified as one of the most recurrently de novo mutated genes in patients with neurodevelopmental disorders (NDDs), including autism spectrum disorder (ASD), intellectual disability and White-Sutton syndrome; however, the neurobiological basis behind these disorders remains unknown. Here, we show that POGZ regulates neuronal development and that ASD-related de novo mutations impair neuronal development in the developing mouse brain and induced pluripotent cell lines from an ASD patient. We also develop the first mouse model heterozygous for a de novo POGZ mutation identified in a patient with ASD, and we identify ASD-like abnormalities in the mice. Importantly, social deficits can be treated by compensatory inhibition of elevated cell excitability in the mice. Our results provide insight into how de novo mutations on high-confidence ASD genes lead to impaired mature cortical network function, which underlies the cellular pathogenesis of NDDs, including ASD.

Contact
Takanobu Nakazawa, Hitoshi Hashimoto , Osaka University, Osaka University , Graduate School of Pharmaceutical Sciences , Graduate School of Pharmaceutical Sciences , Laboratory of Molecular Neuropharmacology , Laboratory of Molecular Neuropharmacology
Contributors

OMERO Dataset
OMERO Project
Source