Detail of Fig3C_IgG



Project
Title
Phase contrast microscopy images of human induced pluripotent stem cells (iPSCs) co-culturing with human primary retinal pigment epithelium (RPE) derived from induced pluripotent stem cell (iPSC-derived RPE) in the presence of control IgG1
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
1.9 MB

Organism
H. sapiens ( NCBI:txid9606 )
Strain(s)
iPS
Cell Line
-

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Phase
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Kanemura et al. (2013) Scientific Reports, 3(2334): 2334.
Related paper(s)

Hoshimi Kanemura, Masahiro J Go, Naoki Nishishita, Noriko Sakai, Hiroyuki Kamao, Yoji Sato, Masayo Takahashi, Shin Kawamata (2013) Pigment epithelium-derived factor secreted from retinal pigment epithelium facilitates apoptotic cell death of iPSC., Scientific reports, Volume 3, pp. 2334

Published in 2013

(Abstract) We show that pigment epithelium-derived factor (PEDF), which is secreted from primary or iPSC-derived retinal pigment epithelium (RPE), dramatically inhibits the growth of iPSCs. PEDF is detected abundantly in culture supernatants of primary or iPSC-derived RPE. Apoptotic cell death is induced in iPSC when co-cultured with RPE, a process that is significantly blocked by addition of antibody against PEDF. Indeed, addition of recombinant PEDF to the iPSC cell culture induces apoptotic cell death in iPSCs, but the expression of pluripotency related-genes is maintained, suggesting that PEDF causes cell death, not differentiation, of iPSCs. To recapitulate this event in vivo, we examined tumor formation in NOG mice after subcutaneous injection of iPSCs with or without an iPSC-derived RPE sheet (2.5 x 10(5) RPE cells). We observed that the tumor forming potential of iPSCs was significantly suppressed by simultaneous transplantation with an iPSC-derived RPE sheet.
(MeSH Terms)

Contact
Shin Kawamata , Foundation for Biomedical Research and Innovation , Research & Development Center for Cell Therapy , Laboratory for Retinal Regeneration
Contributors
Hoshimi Kanemura, Masahiro J. Go, Naoki Nishishita, Noriko Sakai, Hiroyuki Kamao, Yoji Sato, Masayo Takahashi, Shin Kawamata

OMERO Dataset
OMERO Project
Source