Detail of Fig6_Whole_brain_imaging215_20180329_15052



Project
Title
Whole-brain imaging of a 10-week-old C57BL6/J male mouse stained with fluorescent dyes.
Description
Whole-brain imaging of a 10-week-old C57BL6/J male mouse stained with fluorescent dyes.
Release, Updated
2022-11-23
License
CC BY-NC-SA
Kind
Image data
File Formats
.tif
Data size
7.8 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Protein tags
dyes

Datatype
-
Molecular Function (MF)
Biological Process (BP)
brain development ( GO:0007420 )
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
whole-brain imaging
block-face serial microscopy tomography (FAST)
X scale
1.0 micrometer/pixel
Y scale
1.0 micrometer/pixel
Z scale
5 micrometer/pixel
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Seiriki K, et. al. (2019) Nat Protoc, May;14(5):1509-1529.
Related paper(s)

Kaoru Seiriki, Atsushi Kasai, Takanobu Nakazawa, Misaki Niu, Yuichiro Naka, Masato Tanuma, Hisato Igarashi, Kosei Yamaura, Atsuko Hayata-Takano, Yukio Ago, Hitoshi Hashimoto (2019) Whole-brain block-face serial microscopy tomography at subcellular resolution using FAST., Nature protocols, Volume 14, Number 5, pp. 1509-1529

Published in 2019 May (Electronic publication in April 8, 2019, midnight )

(Abstract) Here, we describe an optimized and detailed protocol for block-face serial microscopy tomography (FAST). FAST enables high-speed serial section fluorescence imaging of fixed brains at an axonal spatial resolution and subsequent image data processing. It renders brain-wide anatomical and functional analyses, including structural profiling of nuclear-stained brain at the single-cell level, cell-type-specific mapping with reporter animal brains and neuronal tracing with anterograde/retrograde labeling. Light-sheet fluorescence microscopy of cleared brains is advantageous in regard to imaging speed, but its spatial resolution is generally limited, whereas the opposite is true for conventional confocal microscopy. FAST offers a solution to overcome these technical limitations. This protocol describes detailed procedures for assembling the FAST hardware, sample preparation, imaging and image processing. A single imaging session takes as little as 2.4 h per mouse brain, and sample preparation requires 1 to several days, depending on pretreatments; however, multiple samples can be prepared simultaneously. We anticipate that FAST will contribute to unbiased and hypothesis-free approaches for a better understanding of brain systems.
(MeSH Terms)

Contact
Atsushi Kasai, Hitoshi Hashimoto , Osaka University, Osaka University , Graduate School of Pharmaceutical Sciences , Graduate School of Pharmaceutical Sciences , Laboratory of Molecular Neuropharmacology , Laboratory of Molecular Neuropharmacology
Contributors

OMERO Dataset
OMERO Project
Source