Detail of CycA_video3



Project
Title
Time-lapse microscopy images of tracheal invagination in a D. melanogaster CycA mutant expressing E-cad–GFP and His–RFP
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment
File Formats
Data size
2.1 GB

Organism
D. melanogaster ( NCBI:txid7227 )
Strain(s)
-
Cell Line
-
Gene symbols
CycA
Protein names
E-cad, His
Protein tags
GFP, RFP

Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
NA (tracheal invagination in Drosophila embryos)
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
XY: 0.125 micrometer/pixel, Z: 0.7 micrometer/slice
T scale
3 minute for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV1000
Detector model
PMT detector
Objective model
Olympus, PLAPON 60X O, 60x/1.42
Filter set
-

Summary of Methods
See details in Kondo and Hayashi (2013) Nature, 494, 125-129.
Related paper(s)

Takefumi Kondo, Shigeo Hayashi (2013) Mitotic cell rounding accelerates epithelial invagination., Nature, Volume 494, Number 7435, pp. 125-9

Published in 2013 Feb 7 (Electronic publication in Jan. 13, 2013, midnight )

(Abstract) Mitotic cells assume a spherical shape by increasing their surface tension and osmotic pressure by extensively reorganizing their interphase actin cytoskeleton into a cortical meshwork and their microtubules into the mitotic spindle. Mitotic entry is known to interfere with tissue morphogenetic events that require cell-shape changes controlled by the interphase cytoskeleton, such as apical constriction. However, here we show that mitosis plays an active role in the epithelial invagination of the Drosophila melanogaster tracheal placode. Invagination begins with a slow phase under the control of epidermal growth factor receptor (EGFR) signalling; in this process, the central apically constricted cells, which are surrounded by intercalating cells, form a shallow pit. This slow phase is followed by a fast phase, in which the pit is rapidly depressed, accompanied by mitotic entry, which leads to the internalization of all the cells in the placode. We found that mitotic cell rounding, but not cell division, of the central cells in the placode is required to accelerate invagination, in conjunction with EGFR-induced myosin II contractility in the surrounding cells. We propose that mitotic cell rounding causes the epithelium to buckle under pressure and acts as a switch for morphogenetic transition at the appropriate time.
(MeSH Terms)

Contact
Takefumi Kondo , RIKEN , Center for Developmental Biology , Laboratory for Morphogenetic Signaling
Contributors
Takefumi Kondo, Shigeo Hayashi

OMERO Dataset
OMERO Project
Source