Detail of Fig2A_pten3


Project
SSBD:Repository
Title
Time-lapse TIRF microscopy images of D. discoideum cells expressing PTEN mutants labeled with TMR
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
150.0 MB

Organism
Dictyostelium discoideum ( NCBI:txid44689 )
Strain(s)
-
Cell Line
-
Protein names
PTEN

Datatype
single molecule dynamics
Molecular Function (MF)
Biological Process (BP)
chemotaxis ( GO:0006935 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.033 micrometer/pixel, Z: NA
T scale
33 millisecond for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
TotalInternalReflectionFluorescenceMicroscope
Acquisition mode
TIRF
Contrast method
Fluorescence
Microscope model
OLYMPUS IX-70
Detector model
Andor iXon3
Objective model
OLYMPUS PlanApo 60x 1.45NA Oil TIRFM
Filter set

Summary of Methods
See details in Yasui et al. (2014) PLoS Computational Biology, 10(9): e1003817.
Related paper(s)

Contact
Satomi Matsuoka , Osaka University , Graduate School of Frontier Biosciences , Laboratories for Nanobiology
Contributors
Masato Yasui, Satomi Matsuoka, Masahiro Ueda

OMERO Dataset
OMERO Project
Source