Detail of Fig1c_3D_pelageHF_D0



Project
Title
Phase contrast images of cultured cells isolated from adult mouse pelage HF at day0 in 3D culture.
Description
Phase contrast images of cultured cells isolated from adult mouse pelage HF at day0 in 3D culture.
Release, Updated
2022-11-23
License
CC BY
Kind
Image data
File Formats
.zvi
Data size
2.8 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
hair follicle development ( GO:0001942 )
Cellular Component (CC)
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.3132264 micrometer/pixel
Y scale
0.3132264 micrometer/pixel
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Takeo M, et. al. (2021) Commun Biol., 11(1):1173.
Related paper(s)

Makoto Takeo, Kyosuke Asakawa, Koh-Ei Toyoshima, Miho Ogawa, JingJing Tong, Tarou Irie, Masayuki Yanagisawa, Akio Sato, Takashi Tsuji (2021) Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration., Scientific reports, Volume 11, Number 1, pp. 1173

Published in 2021 Feb 10 (Electronic publication in Feb. 10, 2021, midnight )

(Abstract) In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin beta5 (Itgbeta5)-triple-positive (CD34+/CD49f+/Itgbeta5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy.
(MeSH Terms)

Contact
Takashi Tsuji , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Organ Regeneration
Contributors

OMERO Dataset
OMERO Project
Source