Detail of Fig4A_EGFR-GFP_GRB2


Project
SSBD:Repository
Title
Time-lapse of single-molecule imaging of EGFinduced GRB2–HaloTag::TMR translocation from cytoplasm to the basal plasma membrane.
Description
Time-lapse of single-molecule imaging of EGFinduced GRB2–HaloTag::TMR translocation from cytoplasm to the basal plasma membrane.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
3.5 GB

Organism
Cricetulus griseus ( NCBI:txid10029 )
Strain(s)
-
Cell Line
CHO-K1 ( CLO_0002462 )
Protein names
GRB2

Datatype
-
Molecular Function (MF)
epidermal growth factor-activated receptor activity ( GO:0005006 ) , epidermal growth factor receptor binding ( GO:0005154 )
Biological Process (BP)
epidermal growth factor receptor signaling pathway ( GO:0007173 )
Cellular Component (CC)
Grb2-EGFR complex ( GO:0070436 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
44 nanometer/pixel
Y scale
44 nanometer/pixel
Z scale
-
T scale
30.5 microsecond per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Hiroshima M, et. al. (2018) J Mol Biol., 430(9):1386-1401.
Related paper(s)

Contact
Yasushi Sako , RIKEN , Cellular Informatics Laboratory
Contributors
Michio Hiroshima

OMERO Dataset
OMERO Project
Source