Detail of FIB-SEM490-610_ciliarymembrane



Project
Title
3D FIB-SEM images of a cross section of the ciliary pocket membrane in hTERT-RPE1 cells.
Description
3D FIB-SEM images of a cross section of the ciliary pocket membrane in hTERT-RPE1 cells.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
173.4 MB

Organism
Homo sapiens ( NCBITaxon:9606 )
Strain(s)
-
Cell Line
hTERT RPE-1 cell ( CLO_0004290 )

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
-
Cellular Component (CC)
peroxisome ( GO:0005777 ) ciliary pocket membrane ( GO:0020018 )
Biological Imaging Method
scanning electron microscopy ( Fbbi:00000257 )
X scale
4.13 nanometer/pixel
Y scale
5.25 nanometer/pixel
Z scale
10 nanometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Miyamoto T, et. al. (2020) EMBO J., 39(12):e103499.
Related paper(s)

Tatsuo Miyamoto, Kosuke Hosoba, Takeshi Itabashi, Atsuko H Iwane, Silvia Natsuko Akutsu, Hiroshi Ochiai, Yumiko Saito, Takashi Yamamoto, Shinya Matsuura (2020) Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome., The EMBO journal, Volume 39, Number 12, pp. e103499

Published in 2020 Jun 17 (Electronic publication in May 5, 2020, midnight )

(Abstract) Primary cilia are antenna-like organelles on the surface of most mammalian cells that receive sonic hedgehog (Shh) signaling in embryogenesis and carcinogenesis. Cellular cholesterol functions as a direct activator of a seven-transmembrane oncoprotein called Smoothened (Smo) and thereby induces Smo accumulation on the ciliary membrane where it transduces the Shh signal. However, how cholesterol is supplied to the ciliary membrane remains unclear. Here, we report that peroxisomes are essential for the transport of cholesterol into the ciliary membrane. Zellweger syndrome (ZS) is a peroxisome-deficient hereditary disorder with several ciliopathy-related features and cells from these patients showed a reduced cholesterol level in the ciliary membrane. Reverse genetics approaches revealed that the GTP exchange factor Rabin8, the Rab GTPase Rab10, and the microtubule minus-end-directed kinesin KIFC3 form a peroxisome-associated complex to control the movement of peroxisomes along microtubules, enabling communication between peroxisomes and ciliary pocket membranes. Our findings suggest that insufficient ciliary cholesterol levels may underlie ciliopathies.
(MeSH Terms)

Contact
Tatsuo Miyamoto, Shinya Matsuura , Hiroshima University, Hiroshima University , Department of Genetics and Cell Biology, Research Institute for Radiation Biology and Medicine, Department of Genetics and Cell Biology, Research Institute for Radiation Biology and Medicine
Contributors

OMERO Dataset
OMERO Project
Source