Detail of fig7_myoblastsC2C12_W0.2



Project
Title
Time-course images of mouse skeletal myoblasts C2C12 adhered on a surface with a 0.2mm width cross-linked albumin structure for several days.
Description
Time-course images of mouse skeletal myoblasts C2C12 adhered on a surface with a 0.2mm width cross-linked albumin structure for several days.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
260.2 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
C2C12 ( CLO_0050871 )

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
-
Cellular Component (CC)
-
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
-
Y scale
-
Z scale
-
T scale
1 day per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Shen Y, et. al. (2020) PLoS One., 15(5):e0232518.
Related paper(s)

Yigang Shen, Nobuyuki Tanaka, Hironori Yamazoe, Shunsuke Furutani, Hidenori Nagai, Takayuki Kawai, Yo Tanaka (2020) Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin., PloS one, Volume 15, Number 5, pp. e0232518

Published in 2020 (Electronic publication in May 20, 2020, midnight )

(Abstract) Patterned cell culturing is one of the most useful techniques for understanding the interaction between geometric conditions surrounding cells and their behaviors. The authors previously proposed a simple method for cell patterning with an agarose gel microstructure fabricated by microcasting with a degassed polydimethylsiloxane (PDMS) mold. Although the vacuum pressure produced from the degassed PDMS can drive a highly viscous agarose solution, the influence of solution viscosity on the casting process is unknown. This study investigated the influences of micro-channel dimensions or solution viscosity on the flow of the solution in a micro-channel of a PDMS mold by both experiments and numerical simulation. It was found experimentally that the degassed PDMS mold was able to drive a solution with a viscosity under 575 mPa.s. A simulation model was developed which can well estimate the flow rate in various dimensions of micro-channels. Cross-linked albumin has low viscosity (1 mPa.s) in aqueous solution and can undergo a one-way dehydration process from solution to solid that produces cellular repellency after dehydration. A microstructure of cross-linked albumin was fabricated on a cell culture dish by the microcasting method. After cells were seeded and cultivated on the cell culture dish with the microstructure for 7 days, the cellular pattern of mouse skeletal myoblast cell line C2C12 was observed. The microcasting with cross-linked albumin solution enables preparation of patterned cell culture systems more quickly in comparison with the previous agarose gel casting, which requires a gelation process before the dehydration process.
(MeSH Terms)

Contact
Yo Tanaka , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Integrated Biodevice
Contributors
Yigang Shen,Nobuyuki Tanaka

OMERO Dataset
OMERO Project
Source