Detail of Fig3F_control-Sh

Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in control one.
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in control one.
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Image data
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Mus musculus ( NCBITaxon:10090 )
Cell Line
Protein tags

Molecular Function (MF)
Biological Process (BP)
actin polymerization ( GO:0030041 )
Cellular Component (CC)
dendritic spine ( GO:0043197 )
Biological Imaging Method
two-photon laser scanning microscopy ( Fbbi:00000254 )
X scale
0.103 micrometer/pixel
Y scale
0.103 micrometer/pixel
Z scale
T scale
-20, -10, -1, 1, 15, 30 minutes

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Obashi K, et. al. (2019) Cell Rep., 27(5):1503-1515.
Related paper(s)

Kazuki Obashi, Atsushi Matsuda, Yasuhiro Inoue, Shigeo Okabe (2019) Precise Temporal Regulation of Molecular Diffusion within Dendritic Spines by Actin Polymers during Structural Plasticity., Cell reports, Volume 27, Number 5, pp. 1503-1515.e8

Published in 2019 Apr 30

(Abstract) The biochemical transduction of excitatory synaptic signals occurs in the cytoplasm within dendritic spines. The associated reaction kinetics are shaped by the mobility of the signaling molecules; however, accurate monitoring of diffusional events within the femtoliter-sized spine structures has not yet been demonstrated. Here, we applied two-photon fluorescence correlation spectroscopy and raster image correlation spectroscopy to monitor protein dynamics within spines, revealing that F-actin restricts the mobility of proteins with a molecular mass of >100 kDa. This restriction is transiently removed during actin remodeling at the initial phase of spine structural plasticity. Photobleaching experiments combined with super-resolution imaging indicate that this increase in mobility facilitates molecular interactions, which may modulate the functions of key postsynaptic signaling molecules, such as Tiam1 and CaMKII. Thus, actin polymers in dendritic spines act as precise temporal regulators of molecular diffusion and modulate signal transduction during synaptic plasticity.
(MeSH Terms)

Shigeo Okabe , the University of Tokyo, Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine
Kazuki Obashi

OMERO Dataset
OMERO Project