Detail of Fig2a_WT_180301a_area4



Project
Title
Time-series images of dendritic spines of CA1 pyramidal neurons of WT mice. 
Description
Time-series images of dendritic spines of CA1 pyramidal neurons of WT mice. 
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.oib
Data size
69.1 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
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Molecular Function (MF)
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Biological Process (BP)
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Cellular Component (CC)
dendritic spine ( GO:0043197 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.103 micrometer/pixel
Y scale
0.103 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
20 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
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Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Tanaka S, et. al. (2020) Microscopy (Oxf)., 69(1):44-52.
Related paper(s)

Shinji Tanaka, Yasutaka Masuda, Akihiro Harada, Shigeo Okabe (2020) Impaired actin dynamics and suppression of Shank2-mediated spine enlargement in cortactin knockout mice., Microscopy (Oxford, England), Volume 69, Number 1, pp. 44-52

Published in 2020 Mar 9

(Abstract) Cortactin regulates actin polymerization and stabilizes branched actin network. In neurons, cortactin is enriched in dendritic spines that contain abundant actin polymers. To explore the function of cortactin in dendritic spines, we examined spine morphology and dynamics in cultured neurons taken from cortactin knockout (KO) mice. Histological analysis revealed that the density and morphology of dendritic spines were not significantly different between wild-type (WT) and cortactin KO neurons. Time-lapse imaging of hippocampal slice cultures showed that the extent of spine volume change was similar between WT and cortactin KO neurons. Despite little effect of cortactin deletion on spine morphology and dynamics, actin turnover in dendritic spines was accelerated in cortactin KO neurons. Furthermore, we detected a suppressive effect of cortactin KO on spine head size under the condition of excessive spine enlargement induced by overexpression of a prominent postsynaptic density protein Shank2. These results suggest that cortactin may have a role in maintaining actin organization by stabilizing actin filaments near the postsynaptic density.
(MeSH Terms)

Contact
Shigeo Okabe , University of Tokyo , Department of Cellular Neurobiology, Graduate School of Medicine
Contributors

OMERO Dataset
OMERO Project
Source