Detail of Figure3C_ERK_TracheaDev_E12.5

Time-lapse images of ERK activity in embryonic murine trachea under ex vivo culture condition dissected from E12.5 or E14.5.
Time-lapse images of ERK activity in embryonic murine trachea under ex vivo culture condition dissected from E12.5.
Release, Updated
Image data
File Formats
Data size
43.0 GB

Mus musculus ( NCBITaxon:10090 )
Cell Line
Protein names

Molecular Function (MF)
MAP kinase activity ( GO:0004707 )
Biological Process (BP)
trachea development ( GO:0060438 )
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.994 micrometer/pixel
Y scale
0.994 micrometer/pixel
Z scale
1 micrometer/slice
T scale
5 minutes per time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Yoshida T, et. al. (2020) Front Cell Dev Biol., 8:585640.
Related paper(s)

Takuya Yoshida, Michiyuki Matsuda, Tsuyoshi Hirashima (2020) Incoherent Feedforward Regulation via Sox9 and ERK Underpins Mouse Tracheal Cartilage Development., Frontiers in cell and developmental biology, Volume 8, pp. 585640

Published in 2020 (Electronic publication in Oct. 22, 2020, midnight )

(Abstract) Tracheal cartilage provides architectural integrity to the respiratory airway, and defects in this structure during embryonic development cause severe congenital anomalies. Previous genetic studies have revealed genes that are critical for the development of tracheal cartilage. However, it is still unclear how crosstalk between these proteins regulates tracheal cartilage formation. Here we show a core regulatory network underlying murine tracheal chondrogenesis from embryonic day (E) 12.5 to E15.5, by combining volumetric imaging of fluorescence reporters, inhibitor assays, and mathematical modeling. We focused on SRY-box transcription factor 9 (Sox9) and extracellular signal-regulated kinase (ERK) in the tracheal mesenchyme, and observed a synchronous, inverted U-shaped temporal change in both Sox9 expression and ERK activity with a peak at E14.5, whereas the expression level of downstream cartilage matrix genes, such as collagen II alpha 1 (Col2a1) and aggrecan (Agc1), monotonically increased. Inhibitor assays revealed that the ERK signaling pathway functions as an inhibitory regulator of tracheal cartilage differentiation during this period. These results suggest that expression of the cartilage matrix genes is controlled by an incoherent feedforward loop via Sox9 and ERK, which is supported by a mathematical model. Furthermore, the modeling analysis suggests that a Sox9-ERK incoherent feedforward regulation augments the robustness against the variation of upstream factors. The present study provides a better understanding of the regulatory network underlying the tracheal development and will be helpful for efficient induction of tracheal organoids.

Tsuyoshi Hirashima , Kyoto University , Department of Pathology and Biology of Diseases, Graduate School of Medicine , Multicellular Biophysics in Development and Reproduction

OMERO Dataset
OMERO Project