Detail of Airyscan_hook_BM

High-resolution three-dimensional images of the hook basement membrane in mouse dorsal skin.
High-resolution three-dimensional images of the hook basement membrane in mouse dorsal skin.
Release, Updated
Image data

Mus musculus ( NCBITaxon:10090 )
Cell Line

Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
basement membrane ( GO:0005604 )
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
Image Acquisition
X scale
0.0408118 micrometer/pixel
Y scale
0.0408118 micrometer/pixel
Z scale
0.1804551 micrometer/slice
T scale

Summary of Methods
See details in Tsutsui K, et. al. (2021) Nat Commun., 12(1):2577.
Related paper(s)

Ko Tsutsui, Hiroki Machida, Asako Nakagawa, Kyungmin Ahn, Ritsuko Morita, Kiyotoshi Sekiguchi, Jeffrey H Miner, Hironobu Fujiwara (2021) Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions., Nature communications, Volume 12, Number 1, pp. 2577

Published in 2021 May 10 (Electronic publication in May 10, 2021, midnight )

(Abstract) Inter-tissue interaction is fundamental to multicellularity. Although the basement membrane (BM) is located at tissue interfaces, its mode of action in inter-tissue interactions remains poorly understood, mainly because the molecular and structural details of the BM at distinct inter-tissue interfaces remain unclear. By combining quantitative transcriptomics and immunohistochemistry, we systematically identify the cellular origin, molecular identity and tissue distribution of extracellular matrix molecules in mouse hair follicles, and reveal that BM composition and architecture are exquisitely specialized for distinct inter-tissue interactions, including epithelial-fibroblast, epithelial-muscle and epithelial-nerve interactions. The epithelial-fibroblast interface, namely, hair germ-dermal papilla interface, makes asymmetrically organized side-specific heterogeneity in the BM, defined by the newly characterized interface, hook and mesh BMs. One component of these BMs, laminin alpha5, is required for hair cycle regulation and hair germ-dermal papilla anchoring. Our study highlights the significance of BM heterogeneity in distinct inter-tissue interactions.
(MeSH Terms)

Hironobu Fujiwara , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Tissue Microenvironment

OMERO Dataset
OMERO Project