Detail of Figure1C_nucleolus_3YEAnh4cl



Project
Title
Time-lapse images of nucleolus and spindle pole body (SPB) movements in the sid4-mCherry and Cut11-GFP expressing S. pombe cells cultured in YEA with the presence of NH4CL and 3% glucose
Description
Time-lapse images of nucleolus and spindle pole body (SPB) movements in the sid4-mCherry and Cut11-GFP expressing S. pombe cells cultured in YEA with the presence of NH4CL and 3% glucose
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
uncompressed STK
Data size
7.4 GB

Organism
Schizosaccharomyces pombe ( NCBI:txid4896 )
Strain(s)
-
Cell Line
-
Gene symbols
sid4, Cut11
Protein tags
mCherry, GFP

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
oscillatory muscle contraction ( GO:0014703 )
Cellular Component (CC)
spindle pole body ( GO:0005816 ) nuclear membrane ( GO:0031965 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.16 micrometer/pixel
Y scale
0.16 micrometer/pixel
Z scale
0.2 micrometer/slice
T scale
30 seconds per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Ito H, et. al. (2019) Biochem Biophys Res Commun., 511(4):820-825.
Related paper(s)

Hiroaki Ito, Takeshi Sugawara, Soya Shinkai, Satoshi Mizukawa, Ayaka Kondo, Hisamichi Senda, Kengo Sawai, Koki Ito, Sayaka Suzuki, Masakatsu Takaine, Satoshi Yoshida, Hiromi Imamura, Kenji Kitamura, Toshinori Namba, Shin-Ichi Tate, Masaru Ueno (2019) Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast., Biochemical and biophysical research communications, Volume 511, Number 4, pp. 820-825

Published in 2019 Apr 16 (Electronic publication in March 4, 2019, midnight )

(Abstract) The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affected by different environmental conditions remain poorly understood. In this study, we quantitated movements of the SPB component, which colocalizes with the centromere in fission yeast. We found that SPB movement was significantly reduced at low glucose concentrations. Movement of the SPB was also affected by the presence of ammonium chloride. Power spectral analysis revealed that periodic movement of the SPB is disrupted by low glucose concentrations. Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement. Our results provide novel insight into how SPB movement is regulated by cellular energy status and additional factors such as the medium nutritional composition.
(MeSH Terms)

Contact
Masaru Ueno , Graduate School of Advanced Sciences of Matter, Hiroshima University , Department of Molecular Biotechnology , Ueno Laboratory
Contributors
NA

OMERO Dataset
OMERO Project
Source