Detail of FigureS4_kinetochore_CENPE_20170309_EFcon

Immunostaining images of CENP-E (green), DNA (Hoechst 33342, blue) in normal mouse oocytes during meiosis
Immunostaining images of CENP-E (green), DNA (Hoechst 33342, blue) in normal mouse oocytes during meiosis
Release, Updated
Image data
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Data size
824.2 MB

Mus musculus ( NCBITaxon:10090 )
Cell Line
Protein names

Molecular Function (MF)
Biological Process (BP)
nuclear envelope breakdown ( GO:0051081 ) mitotic cell cycle ( GO:0007067 ) cytoplasm organization ( GO:0007028 )
Cellular Component (CC)
kinetochore microtubule ( GO:0005828 ) chromosome ( GO:0005694 )
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
0.0830266 micrometer/pixel
Y scale
0.0830266 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Kyogoku H, et. al. (2017) Dev. Cell, 41(3): 287-298.
Related paper(s)

Hirohisa Kyogoku, Tomoya S Kitajima (2017) Large Cytoplasm Is Linked to the Error-Prone Nature of Oocytes., Developmental cell, Volume 41, Number 3, pp. 287-298.e4

Published in 2017 May 8

(Abstract) Chromosome segregation during meiosis in oocytes is error prone. The uniquely large cytoplasmic size of oocytes, which provides support for embryogenesis after fertilization, might be a predisposing factor for meiotic errors. However, this hypothesis remains unproven. Here, we show that cytoplasmic size affects the functionality of the acentrosomal spindle. Artificially decreasing the cytoplasmic size in mouse oocytes allows the acentrosomal spindle poles to have a better-focused distribution of microtubule-organizing centers and to biorient chromosomes more efficiently, whereas enlargement of the cytoplasmic size has the opposite effects. Moreover, we found that the cytoplasmic size-dependent dilution of nuclear factors, including anaphase inhibitors that are preformed at the nuclear membrane, limits the spindle's capacity to prevent anaphase entry with misaligned chromosomes. The present study defines a large cytoplasmic volume as a cell-intrinsic feature linked to the error-prone nature of oocytes. This may represent a trade-off between meiotic fidelity and post-fertilization developmental competence.
(MeSH Terms)

Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation

OMERO Dataset
OMERO Project