Detail of Figure6G_2014_4_24

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Live cell imaging of Mad1-mNeonGreen-CENP-C and H2B-mCherry expressing mouse oocytes during meiosis
Live cell imaging of Mad1-mNeonGreen-CENP-C and H2B-mCherry expressing mouse oocytes during meiosis
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Image data
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Data size
68.6 GB

Mus musculus ( NCBITaxon:10090 )
Cell Line

Molecular Function (MF)
Biological Process (BP)
nuclear envelope breakdown ( GO:0051081 ) mitotic cell cycle ( GO:0007067 ) cytoplasm organization ( GO:0007028 )
Cellular Component (CC)
kinetochore microtubule ( GO:0005828 ) chromosome ( GO:0005694 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.1186094 micrometer/pixel
Y scale
0.1186094 micrometer/pixel
Z scale
1.5 micrometer/slice
T scale
3 minutes for each time interval

Image Acquisition
Experiment type
Microscope type
Acquisition mode
Contrast method
Microscope model
Detector model
Objective model
Filter set

Summary of Methods
See details in Kyogoku H, et. al. (2017) Dev. Cell, 41(3): 287-298.
Related paper(s)

Hirohisa Kyogoku, Tomoya S Kitajima (2017) Large Cytoplasm Is Linked to the Error-Prone Nature of Oocytes., Developmental cell, Volume 41, Number 3, pp. 287-298.e4

Published in 2017 May 8

(Abstract) Chromosome segregation during meiosis in oocytes is error prone. The uniquely large cytoplasmic size of oocytes, which provides support for embryogenesis after fertilization, might be a predisposing factor for meiotic errors. However, this hypothesis remains unproven. Here, we show that cytoplasmic size affects the functionality of the acentrosomal spindle. Artificially decreasing the cytoplasmic size in mouse oocytes allows the acentrosomal spindle poles to have a better-focused distribution of microtubule-organizing centers and to biorient chromosomes more efficiently, whereas enlargement of the cytoplasmic size has the opposite effects. Moreover, we found that the cytoplasmic size-dependent dilution of nuclear factors, including anaphase inhibitors that are preformed at the nuclear membrane, limits the spindle's capacity to prevent anaphase entry with misaligned chromosomes. The present study defines a large cytoplasmic volume as a cell-intrinsic feature linked to the error-prone nature of oocytes. This may represent a trade-off between meiotic fidelity and post-fertilization developmental competence.
(MeSH Terms)

Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation

OMERO Dataset
OMERO Project