Detail of FigureS7A_131026_MT_ACA_AZD



Project
Title
Timelapse confocal images of oocytes treated with 500 nM AZD1152 before meiotic resumption were stained for MTs (green), KTs (ACA, red) and chromosomes (Hoechst33342, blue) after cold treatment
Description
Timelapse confocal images of oocytes treated with 500 nM AZD1152 before meiotic resumption were stained for MTs (green), KTs (ACA, red) and chromosomes (Hoechst33342, blue) after cold treatment
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
903.8 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Protein names
ACA

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
mitotic cell cycle ( GO:0007067 )
Cellular Component (CC)
kinetochore microtubule ( GO:0005828 ) chromosome ( GO:0005694 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.0593047 micrometer/pixel
Y scale
0.0593047 micrometer/pixel
Z scale
0.25 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Yoshida S, et. al. (2015) Dev Cell., 33(5):589-602.
Related paper(s)

Shuhei Yoshida, Masako Kaido, Tomoya S Kitajima (2015) Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes., Developmental cell, Volume 33, Number 5, pp. 589-602

Published in 2015 Jun 8 (Electronic publication in May 28, 2015, midnight )

(Abstract) A model for mitosis suggests that correct kinetochore-microtubule (KT-MT) attachments are stabilized by spatial separation of the attachment sites from Aurora B kinase through sister KT stretching. However, the spatiotemporal regulation of attachment stability during meiosis I (MI) in oocytes remains unclear. Here, we found that in mouse oocytes, Aurora B and C (B/C) are located in close proximity to KT-MT attachment sites after bivalent stretching due to an intrinsic property of the MI chromosomes. The Aurora B/C activity destabilizes correct attachments while allowing a considerable amount of incorrect attachments to form. KT-MT attachments are eventually stabilized through KT dephosphorylation by PP2A-B56 phosphatase, which is progressively recruited to KTs depending on the BubR1 phosphorylation resulting from the timer Cdk1 and independent of bivalent stretching. Thus, oocytes lack a mechanism for coordinating bivalent stretching and KT phosphoregulation during MI, which may explain the high frequency of KT-MT attachment errors.
(MeSH Terms)

Contact
Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation
Contributors

OMERO Dataset
OMERO Project
Source