Detail of FigureS2AB_150123_tdTomato-CENP-C_Hec1-mEGFP_Nuf_5h_L8



Project
Title
Timelapse confocal images of oocytes expressing Hec1-mEGFP (green) and tdTomato-CENP-C (red) at 6 hours after NEBD during MI
Description
Timelapse confocal images of oocytes expressing Hec1-mEGFP (green) and tdTomato-CENP-C (red) at 6 hours after NEBD during MI
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
1.7 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
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Cell Line
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Gene symbols
Hec1
Protein names
CENP-C
Protein tags
tdTomato

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
mitotic cell cycle ( GO:0007067 )
Cellular Component (CC)
kinetochore microtubule ( GO:0005828 ) chromosome ( GO:0005694 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.0527153 micrometer/pixel
Y scale
0.0527153 micrometer/pixel
Z scale
0.5 micrometer/slice
T scale
1 minute for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Yoshida S, et. al. (2015) Dev Cell., 33(5):589-602.
Related paper(s)

Shuhei Yoshida, Masako Kaido, Tomoya S Kitajima (2015) Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes., Developmental cell, Volume 33, Number 5, pp. 589-602

Published in 2015 Jun 8 (Electronic publication in May 28, 2015, midnight )

(Abstract) A model for mitosis suggests that correct kinetochore-microtubule (KT-MT) attachments are stabilized by spatial separation of the attachment sites from Aurora B kinase through sister KT stretching. However, the spatiotemporal regulation of attachment stability during meiosis I (MI) in oocytes remains unclear. Here, we found that in mouse oocytes, Aurora B and C (B/C) are located in close proximity to KT-MT attachment sites after bivalent stretching due to an intrinsic property of the MI chromosomes. The Aurora B/C activity destabilizes correct attachments while allowing a considerable amount of incorrect attachments to form. KT-MT attachments are eventually stabilized through KT dephosphorylation by PP2A-B56 phosphatase, which is progressively recruited to KTs depending on the BubR1 phosphorylation resulting from the timer Cdk1 and independent of bivalent stretching. Thus, oocytes lack a mechanism for coordinating bivalent stretching and KT phosphoregulation during MI, which may explain the high frequency of KT-MT attachment errors.
(MeSH Terms)

Contact
Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation
Contributors

OMERO Dataset
OMERO Project
Source