Detail of FigS3A_171231_MII_monastrol_cenpc_PIASdn_image19



Project
Title
Immunostaining images of PIAS1(dn)-CENP-C expressing mouse oocytes cultured with monastrol at telophase I (kinetochores: green, DNA: blue)
Description
Immunostaining images of PIAS1(dn)-CENP-C expressing mouse oocytes cultured with monastrol at telophase I (kinetochores: green, DNA: blue)
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
11.6 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-
Protein names
CENP-C, PIAS1

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
centromeric sister chromatid cohesion ( GO:0070601 ) meiotic cell cycle ( GO:0051321 )
Cellular Component (CC)
kinetochore ( GO:0000776 ) chromosome ( GO:0005694 )
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
0.0593047 micrometer/pixel
Y scale
0.0593047 micrometer/pixel
Z scale
1 micrometer/slice
T scale
NA

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Ding Y, et. al. (2018) Current Biology, 28(10): 1661-1669.
Related paper(s)

Yi Ding, Masako Kaido, Elena Llano, Alberto M Pendas, Tomoya S Kitajima (2018) The Post-anaphase SUMO Pathway Ensures the Maintenance of Centromeric Cohesion through Meiosis I-II Transition in Mammalian Oocytes., Current biology : CB, Volume 28, Number 10, pp. 1661-1669.e4

Published in 2018 May 21 (Electronic publication in May 10, 2018, midnight )

(Abstract) The production of haploid gametes requires the maintenance of centromeric cohesion between sister chromatids through the transition between two successive meiotic divisions, meiosis I and meiosis II. One mechanism for the cohesion maintenance is shugoshin-dependent protection of centromeric cohesin at anaphase I onset [1-3]. However, how centromeric cohesion is maintained during late anaphase I and telophase I, when centromeric shugoshin is undetectable [1-3], remains largely unexplored. Here we show that the centromeric small ubiquitin-related modifier (SUMO) pathway is critical for the maintenance of centromeric cohesion during post-anaphase-I periods in mouse oocytes. SUMO2/3 and E3 ligase PIAS are enriched near centromeres during late anaphase I and telophase I. Specific perturbation of the centromeric SUMO pathway results in precocious loss of centromeric cohesin at telophase I, although shugoshin-dependent centromeric protection at anaphase I onset remains largely intact. Prevention of the SUMO perturbation during post-anaphase-I periods restores the maintenance of centromeric cohesion through the meiosis I-II transition. Thus, the post-anaphase-I centromeric SUMO pathway ensures continuous maintenance of centromeric cohesion through the meiosis I-II transition.
(MeSH Terms)

Contact
Tomoya S. Kitajima , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Chromosome Segregation
Contributors

OMERO Dataset
OMERO Project
Source