Detail of figure1D_NuCyM_posi3_3



Project
Title
Images of cell morphology development during MDCK mitosis
Description
Images of cell morphology development during MDCK mitosis
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
59.7 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
MDCK cell ( CLO_0007646 )

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
-
Cellular Component (CC)
plasma membrane ( GO:0005886 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.132 micrometer/pixel
Y scale
0.132 micrometer/pixel
Z scale
2 micrometer/slice
T scale
1 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Imanishi A, et. al. (2018) Cell Struct. Funct., 43(2): 129-140.
Related paper(s)

Ayako Imanishi, Tomokazu Murata, Masaya Sato, Kazuhiro Hotta, Itaru Imayoshi, Michiyuki Matsuda, Kenta Terai (2018) A Novel Morphological Marker for the Analysis of Molecular Activities at the Single-cell Level., Cell structure and function, Volume 43, Number 2, pp. 129-140

Published in 2018 Aug 10 (Electronic publication in June 29, 2018, midnight )

(Abstract) For more than a century, hematoxylin and eosin (H&E) staining has been the de facto standard for histological studies. Consequently, the legacy of histological knowledge is largely based on H&E staining. Due to the recent advent of multi-photon excitation microscopy, the observation of live tissue is increasingly being used in many research fields. Adoption of this technique has been further accelerated by the development of genetically encoded biosensors for ions and signaling molecules. However, H&E-based histology has not yet begun to fully utilize in vivo imaging due to the lack of proper morphological markers. Here, we report a genetically encoded fluorescent marker, NuCyM (Nucleus, Cytosol, and Membrane), which is designed to recapitulate H&E staining patterns in vivo. We generated a transgenic mouse line ubiquitously expressing NuCyM by using a ROSA26 bacterial artificial chromosome (BAC) clone. NuCyM evenly marked the plasma membrane, cytoplasm and nucleus in most tissues, yielding H&E staining-like images. In the NuCyM-expressing cells, cell division of a single cell was clearly observed as five basic phases during M phase by three-dimensional imaging. We next crossed NuCyM mice with transgenic mice expressing an ERK biosensor based on the principle of Forster resonance energy transfer (FRET). Using NuCyM, ERK activity in each cell could be extracted from the FRET images. To further accelerate the image analysis, we employed machine learning-based segmentation methods, and thereby automatically quantitated ERK activity in each cell. In conclusion, NuCyM is a versatile cell morphological marker that enables us to grasp histological information as with H&E staining.Key words: in vivo imaging, histology, machine learning, molecular activity.
(MeSH Terms)

Contact
Kenta Terai , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors

OMERO Dataset
OMERO Project
Source