Detail of SuppFig4B_20171022_ErbB2i_Post1



Project
Title
Image of ERK activity in EKAREV-NLS organoids cultured in the R-spondin 1-depleted (EN) media treated with 10uM ErbB2 inhibitor (CP-724714)
Description
Image of ERK activity in EKAREV-NLS organoids cultured in the R-spondin 1-depleted (EN) media treated with 10uM ErbB2 inhibitor (CP-724714)
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
Data size
10.9 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
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Gene symbols
ErbB2
Protein names
ERK

Datatype
-
Molecular Function (MF)
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Biological Process (BP)
activation of MAPERK kinase ( GO:0000186 ) epidermal growth factor signaling pathway ( GO:0007173 ) ERBB2 signaling pathway ( GO:0038128 ) ERBB2-EGFR signaling pathway ( GO:0038134 )
Cellular Component (CC)
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Biological Imaging Method
X scale
0.497 micrometer/pixel
Y scale
0.497 micrometer/pixel
Z scale
4 micrometer/slice
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
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Microscope model
-
Detector model
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Objective model
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Filter set
-

Summary of Methods
See details in Muta Y, et. al. (2018) Nat. Commun., 9(1): 2174.
Related paper(s)

Yu Muta, Yoshihisa Fujita, Kenta Sumiyama, Atsuro Sakurai, M Mark Taketo, Tsutomu Chiba, Hiroshi Seno, Kazuhiro Aoki, Michiyuki Matsuda, Masamichi Imajo (2018) Composite regulation of ERK activity dynamics underlying tumour-specific traits in the intestine., Nature communications, Volume 9, Number 1, pp. 2174

Published in 2018 Jun 5 (Electronic publication in June 5, 2018, midnight )

(Abstract) Acting downstream of many growth factors, extracellular signal-regulated kinase (ERK) plays a pivotal role in regulating cell proliferation and tumorigenesis, where its spatiotemporal dynamics, as well as its strength, determine cellular responses. Here, we uncover the ERK activity dynamics in intestinal epithelial cells (IECs) and their association with tumour characteristics. Intravital imaging identifies two distinct modes of ERK activity, sustained and pulse-like activity, in IECs. The sustained and pulse-like activities depend on ErbB2 and EGFR, respectively. Notably, activation of Wnt signalling, the earliest event in intestinal tumorigenesis, augments EGFR signalling and increases the frequency of ERK activity pulses through controlling the expression of EGFR and its regulators, rendering IECs sensitive to EGFR inhibition. Furthermore, the increased pulse frequency is correlated with increased cell proliferation. Thus, ERK activity dynamics are defined by composite inputs from EGFR and ErbB2 signalling in IECs and their alterations might underlie tumour-specific sensitivity to pharmacological EGFR inhibition.
(MeSH Terms)

Contact
Masamichi Imajo , Kyoto University , Graduate School of Biostudies , Laboratory of Bioimaging and Cell Signaling
Contributors

OMERO Dataset
OMERO Project
Source