Detail of Fig1b_movie1_ECNuclei_trajectory



Project
Title
Time-lapse images of tracking of individual EC nuclei in an elongating branch.
Description
Time-lapse images of tracking of individual EC nuclei in an elongating branch.
Release, Updated
2021-09-30
License
CC BY
Kind
Image data
File Formats
.tif
Data size
1.5 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
endothelial cell ( CLO_0000115 )

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
endothelial cell morphogenesis ( GO:0001886 )
Cellular Component (CC)
nucleus ( GO:0005634 )
Biological Imaging Method
time lapse microscopy ( Fbbi:00000249 )
X scale
0.83 micrometer/pixel
Y scale
0.83 micrometer/pixel
Z scale
-
T scale
3 minutes per time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Takubo N, et. al. (2019) Sci Rep, 9(1):9304.
Related paper(s)

Naoko Takubo, Fumitaka Yura, Kazuaki Naemura, Ryo Yoshida, Terumasa Tokunaga, Tetsuji Tokihiro, Hiroki Kurihara (2019) Cohesive and anisotropic vascular endothelial cell motility driving angiogenic morphogenesis., Scientific reports, Volume 9, Number 1, pp. 9304

Published in 2019 Jun 26 (Electronic publication in June 26, 2019, midnight )

(Abstract) Vascular endothelial cells (ECs) in angiogenesis exhibit inhomogeneous collective migration called "cell mixing", in which cells change their relative positions by overtaking each other. However, how such complex EC dynamics lead to the formation of highly ordered branching structures remains largely unknown. To uncover hidden laws of integration driving angiogenic morphogenesis, we analyzed EC behaviors in an in vitro angiogenic sprouting assay using mouse aortic explants in combination with mathematical modeling. Time-lapse imaging of sprouts extended from EC sheets around tissue explants showed directional cohesive EC movements with frequent U-turns, which often coupled with tip cell overtaking. Imaging of isolated branches deprived of basal cell sheets revealed a requirement of a constant supply of immigrating cells for ECs to branch forward. Anisotropic attractive forces between neighboring cells passing each other were likely to underlie these EC motility patterns, as evidenced by an experimentally validated mathematical model. These results suggest that cohesive movements with anisotropic cell-to-cell interactions characterize the EC motility, which may drive branch elongation depending on a constant cell supply. The present findings provide novel insights into a cell motility-based understanding of angiogenic morphogenesis.
(MeSH Terms)

Contact
Naoko Takubo, Hiroki Kurihara , The University of Tokyo, The University of Tokyo , Present address: Isotope Science Center, Department of Molecular Cell Biology, Graduate School of Medicine
Contributors
Naoko Takubo, Hiroki Kurihara, Ryo Yoshida, Terumasa Tokunaga

OMERO Dataset
OMERO Project
Source