Detail of Figure2C_dorsal_24ss



Project
Title
Immunostaining image of transplanted cells Tg(b-actin:DsRed) into a Tg(zic1:GFP) host medaka embryo originally located at 24 somite stage and differentiated into HBCs
Description
Immunostaining image of transplanted cells Tg(b-actin:DsRed) into a Tg(zic1:GFP) host medaka embryo originally located at 24 somite stage and differentiated into HBCs
Release, Updated
2020-12-23
License
CC BY
Kind
Image data based on NA
File Formats
bmp
Data size
1.2 MB

Organism
Oryzias ( NCBI:txid8089 )
Strain(s)
Medaka: Tg(b-actin:DsRed
Cell Line
-
Gene symbols
zic1
Protein names
b-actin
Protein tags
GFP
Reporter
DsRed

Datatype
NA
Molecular Function (MF)
somite development ( GO:0061053 )
Biological Process (BP)
somite development ( GO:0061053 )
Cellular Component (CC)
antibody ( GO:0019814 )
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
antibody ( Fbbi:00000401 )
X scale
-
Y scale
-
Z scale
-
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Abe K, et. al. (2019) Cell Rep., 27(3):928-939.
Related paper(s)

Kota Abe, Atsuko Shimada, Sayaka Tayama, Hotaka Nishikawa, Takuya Kaneko, Sachiko Tsuda, Akari Karaiwa, Takaaki Matsui, Tohru Ishitani, Hiroyuki Takeda (2019) Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite., Cell reports, Volume 27, Number 3, pp. 928-939.e4

Published in 2019 Apr 16

(Abstract) Establishment of robust gene expression boundary is crucial for creating elaborate morphology during development. However, mechanisms underlying boundary formation have been extensively studied only in a few model systems. We examined the establishment of zic1/zic4-expression boundary demarcating dorsoventral boundary of the entire trunk of medaka fish (Oryzias latipes) and identified a subgroup of dermomyotomal cells called horizontal boundary cells (HBCs) as crucial players for the boundary formation. Embryological and genetic analyses demonstrated that HBCs play crucial roles in the two major events of the process, i.e., refinement and maintenance. In the refinement, HBCs could serve as a chemical barrier against Wnts from the neural tube by expressing Hhip. At later stages, HBCs participate in the maintenance of the boundary by differentiating into the horizontal myoseptum physically inhibiting cell mixing across the boundary. These findings reveal the mechanisms underlying the dorsoventral boundary in the teleost trunk by specialized boundary cells.
(MeSH Terms)

Contact
Hiroyuki Takeda , Graduate School of Science, University of Tokyo , Department of Biological Sciences
Contributors
NA

OMERO Dataset
OMERO Project
Source