Detail of Fig2d_FIB-SEM_spine_synapse



Project
Title
FIB-SEM image of spine synapse profiles in wild-type (C57BL/6J) mice
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
3.0 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
synapse morphology
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
neuron spine ( GO:0044309 ) spine synapse ( GO:0106033 )
Biological Imaging Method
XYZ Scale
XY: 5.58 nanometre/pixel; Z:NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
Other
Contrast method
Other
Microscope model
ZEISS CrossBeam 540
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Sato et al. (2019) Microscopy (Oxf), 68(2): 122-132.
Related paper(s)

Yuka Sato, Shigeo Okabe (2019) Nano-scale analysis of synapse morphology in an autism mouse model with 15q11-13 copy number variation using focused ion beam milling and scanning electron microscopy., Microscopy (Oxford, England), Volume 68, Number 2, pp. 122-132

Published in 2019 Apr 1

(Abstract) Circuit-level alternations in patients of autism spectrum disorder (ASD) is under active investigation and detailed characterization of synapse morphology in ASD model mice should be informative. We utilized focused ion beam milling and scanning electron microscopy (FIB-SEM) to obtain three-dimensional images of synapses in the layer 2/3 of the somatosensory cortex from a mouse model for ASD with human 15q11-13 chromosomal duplication (15q dup mice). We found a trend of higher spine density and a higher fraction of astrocytic contact with both spine and shaft synapses in 15q dup mice. Measurement of spine synapse structure indicated that the size of the post-synaptic density (PSD), spine head volume, spine head width and spine neck width were smaller in 15q dup mice. Categorization of spine synapses into five classes suggested a trend of less frequent mushroom spines in 15q dup mice. These results suggest relative increase in excitatory synapses with immature morphology but more astrocytic contacts in 15q dup mice, which may be linked to enhanced synapse turnover seen in ASD mouse models.
(MeSH Terms)

Contact
Shigeo Okabe , Graduate School of Medicine, University of Tokyo , Department of Cellular Neurobiology , Okabe Laboratory
Contributors
Yuka Sato, Shigeo Okabe

OMERO Dataset
OMERO Project
Source