Detail of Figure3A_EM_WT_Dorsal



Project
Title
Electron micrographs of the dorsal areas of choroidal vessels from wild-type mice
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
1.0 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
Electron micrographs
Molecular Function (MF)
Biological Process (BP)
retinal pigment epithelium development ( GO:0003406 )
Cellular Component (CC)
photoreceptor cell cilium ( GO:0097733 )
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
BrightField
Contrast method
Brightfield
Microscope model
JEM 1010 transmission electon microscope
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Goto et al. (2018) eLife, 7: e32358.
Related paper(s)

So Goto, Akishi Onishi, Kazuyo Misaki, Shigenobu Yonemura, Sunao Sugita, Hiromi Ito, Yoko Ohigashi, Masatsugu Ema, Hirokazu Sakaguchi, Kohji Nishida, Masayo Takahashi (2018) Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells., eLife, Volume 7

Published in 2018 Apr 3 (Electronic publication in April 3, 2018, midnight )

(Abstract) VEGF secreted from retinal pigment epithelial (RPE) cells is responsible for the choroidal vascular development; however, the molecular regulatory mechanism is unclear. We found that Aldh1a1(-/-) mice showed choroidal hypoplasia with insufficient vascularization in the dorsal region, although Aldh1a1, an enzyme that synthesizes retinoic acids (RAs), is expressed in the dorsal neural retina, not in the RPE/choroid complex. The level of VEGF in the RPE/choroid was significantly decreased in Aldh1a1(-/-) mice, and RA-dependent enhancement of VEGF was observed in primary RPE cells. An RA-deficient diet resulted in dorsal choroidal hypoplasia, and simple RA treatment of Aldh1a1(-/-) pregnant females suppressed choroid hypoplasia in their offspring. We also found downregulation of Sox9 in the dorsal neural retina and RPE of Aldh1a1(-/-) mice and RPE-specific disruption of Sox9 phenocopied Aldh1a1(-/-) choroidal development. These results suggest that RAs produced by Aldh1a1 in the neural retina directs dorsal choroidal vascular development via Sox9 upregulation in the dorsal RPE cells to enhance RPE-derived VEGF secretion.
(MeSH Terms)

Contact
Akishi Onishi , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Retinal Regeneration
Contributors
So Goto, Akishi Onishi, Kazuyo Misaki, Shigenobu Yonemura, Sunao Sugita, Hiromi Ito, Yoko Ohigashi, Masatsugu Ema, Hirokazu Sakaguchi, Kohji Nishida, Masayo Takahashi

OMERO Dataset
OMERO Project
Source