Detail of zebrafish_in_toto_wt.mat




Project
Title
BDML file for in toto reconstruction of zebrafish wild-type development
Description
NA
Release, Updated
2013-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Quantitative data based on Experiment

Organism
D. rerio ( NCBITaxon:7955 )
Strain(s)
-
Cell Line
-

Datatype
nuclear positions
Molecular Function (MF)
Biological Process (BP)
embryo development ( GO:0009790 )
Cellular Component (CC)
nucleus ( GO:0005634 )
Biological Imaging Method
Image Acquisition
ConfocalMicroscope , TimeLapse , Other , Other
XYZ Scale
XY: 1.0 micrometer/pixel, Z: 1.0 micrometer/frame
T scale
90 second for each time interval

Summary of Methods
See details in Keller et al. (2008) Science, 322(5904): 1065-1069.
Related paper(s)

Philipp J Keller, Annette D Schmidt, Joachim Wittbrodt, Ernst H K Stelzer (2008) Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy., Science (New York, N.Y.), Volume 322, Number 5904, pp. 1065-9

Published in 2008 Nov 14 (Electronic publication in Oct. 9, 2008, midnight )

(Abstract) A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.
(MeSH Terms)

Contact
Philipp J. Keller , European Molecular Biology Laboratory , Cell Biology and Biophysics Unit
Contributors
Philipp J. Keller, Annette D. Schmidt, Joachim Wittbrodt, Ernst H.K. Stelzer

Local ID
zebrafish_in_toto_wt.mat
BDML ID
None
BDML/BD5
Workflow https://ssbd.riken.jp/data/pdpml/5-Keller-FishEmbryo_pdpml1.0.xml (md5)