Detail of 5E3rd4hrSTMSGlc-1_Render_SeriesR



Project
Title
Immunostaing confocal images of glycogen in the body wall muscules with anti-glycogen antibodies in drosophila under starvation for 4 hours
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
5.6 MB

Organism
Drosophila ( NCBI:txid7215 )
Strain(s)
-
Cell Line
-

Datatype
tissuel immunostaining
Molecular Function (MF)
Biological Process (BP)
glycogen breakdown ( GO:0005980 )
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Zeiss LSM700
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Yamada et al. (2018) Development, 145(6): dev158865.
Related paper(s)

Takayuki Yamada, Okiko Habara, Hitomi Kubo, Takashi Nishimura (2018) Fat body glycogen serves as a metabolic safeguard for the maintenance of sugar levels in Drosophila., Development (Cambridge, England), Volume 145, Number 6

Published in 2018 Mar 14 (Electronic publication in March 14, 2018, midnight )

(Abstract) Adapting to changes in food availability is a central challenge for survival. Glucose is an important resource for energy production, and therefore many organisms synthesize and retain sugar storage molecules. In insects, glucose is stored in two different forms: the disaccharide trehalose and the branched polymer glycogen. Glycogen is synthesized and stored in several tissues, including in muscle and the fat body. Despite the major role of the fat body as a center for energy metabolism, the importance of its glycogen content remains unclear. Here, we show that glycogen metabolism is regulated in a tissue-specific manner under starvation conditions in the fruit fly Drosophila The mobilization of fat body glycogen in larvae is independent of Adipokinetic hormone (Akh, the glucagon homolog) but is regulated by sugar availability in a tissue-autonomous manner. Fat body glycogen plays a crucial role in the maintenance of circulating sugars, including trehalose, under fasting conditions. These results demonstrate the importance of fat body glycogen as a metabolic safeguard in Drosophila.
(MeSH Terms)

Contact
Takashi Nishimura , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Growth Control Signaling
Contributors
Takayuki Yamada, Okiko Habara, Hitomi Kubo, Takashi Nishimura

OMERO Dataset
OMERO Project
Source