Detail of fig3c_sh8704T1_PMN


Project
SSBD:Repository
Title
Time-lapse images of osteoopontin (OPN)-depleted 4T1 cells expressing sh870 injected into BALB/c mice
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
347.7 MB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
PMN expressing ERK biosensor and 4T1 tumor cells expressing Red fluorescent protein
Cell Line
-

Datatype
Cell dynamics, ERK activation by FRET
Molecular Function (MF)
Biological Process (BP)
activation of MAPKK activity ( GO:0007555 )
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
XY: 0.662 micrometer/pixel, Z: 1 micrometer/slice
T scale
60.31 second for each time interval

Image Acquisition
Experiment type
FRET
Microscope type
MultiPhotonMicroscopy
Acquisition mode
-
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-BX61WI
Detector model
-
Objective model
Olympus XLPLN 25XW-MP
Filter set

Summary of Methods
See details in Kamioka et al. (2017) Cancer Sci, 108(2): 226-235.
Related paper(s)

Contact
Yuji Kamioka , Kansai Medical University
Contributors
Yuji Kamioka, Kanako Takakura, Kenta Sumiyama, Michiyuki Matsuda

OMERO Dataset
OMERO Project
Source