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Key
Organism
Strain
Cell Line
Gene Symbol
Protein name
Protein tag
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Gene Ontology
Biological Imaging Methods
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Detail of fig3c_sh8704T1_PMN
SSBD:OMERO.gallery Dataset
SSBD:OMERO Dataset
SSBD:OMERO.gallery Project
SSBD:OMERO Project
4D View for Quantitative Data
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SSBD:downloads
Project
113-Kamioka-CellDyn
SSBD:Repository
ssbd-repos-000113
Title
Time-lapse images of osteoopontin (OPN)-depleted 4T1 cells expressing sh870 injected into BALB/c mice
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
347.7 MB
Organism
Mus musculus
(
NCBI:txid10090
)
Strain(s)
PMN expressing ERK biosensor and 4T1 tumor cells expressing Red fluorescent protein
Cell Line
-
Datatype
Cell dynamics, ERK activation by FRET
Molecular Function (MF)
Biological Process (BP)
activation of MAPKK activity (
GO:0007555
)
Cellular Component (CC)
cell (
GO:0005623
)
Biological Imaging Method
XYZ Scale
XY: 0.662 micrometer/pixel, Z: 1 micrometer/slice
T scale
60.31 second for each time interval
Image Acquisition
Experiment type
FRET
Microscope type
MultiPhotonMicroscopy
Acquisition mode
-
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-BX61WI
Detector model
-
Objective model
Olympus XLPLN 25XW-MP
Filter set
Summary of Methods
See details in Kamioka et al. (2017) Cancer Sci, 108(2): 226-235.
Related paper(s)
Contact
Yuji Kamioka , Kansai Medical University
Contributors
Yuji Kamioka, Kanako Takakura, Kenta Sumiyama, Michiyuki Matsuda
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-1416
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-260
Source
https://ssbd.riken.jp/data/113-Kamioka-CellDyn/source/fig3c_sh8704T1_PMN.zip
(205.3 MB)