Detail of fig6A_cryosection



Project
Title
Immunoelectron microscop image of nakanori distribution on cryosection of HeLa cells
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
10.8 MB

Organism
H. sapiens ( NCBI:txid9606 )
Strain(s)
HeLa
Cell Line
-

Datatype
membrane nakanori distribution
Molecular Function (MF)
Biological Process (BP)
protein lipid complex assembly ( GO:0065005 )
Cellular Component (CC)
protein lipid complex ( GO:0032994 ) plasma membrane ( GO:0005886 )
Biological Imaging Method
XYZ Scale
XY: 0.83 nanometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
BrightField
Contrast method
Brightfield
Microscope model
JEOL1200 EX-II, JEM1230
Detector model
Imaging Plate and Fujifilm FDL5000, Olympus-SIS Veleta
Objective model
-
Filter set
-

Summary of Methods
See details in Makino et al. (2016) FASEB J, 31(4): 1301-1322.
Related paper(s)

Asami Makino, Mitsuhiro Abe, Reiko Ishitsuka, Motohide Murate, Takuma Kishimoto, Shota Sakai, Francoise Hullin-Matsuda, Yukiko Shimada, Takehiko Inaba, Hideyuki Miyatake, Hideko Tanaka, Atsushi Kurahashi, Chan-Gi Pack, Rinshi S Kasai, Shuku Kubo, Nicole L Schieber, Naoshi Dohmae, Naoya Tochio, Kyoji Hagiwara, Yutaka Sasaki, Yoko Aida, Fumihiro Fujimori, Takanori Kigawa, Kozo Nishibori, Robert G Parton, Akihiro Kusumi, Yasushi Sako, Gregor Anderluh, Makoto Yamashita, Tetsuyuki Kobayashi, Peter Greimel, Toshihide Kobayashi (2017) A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C., FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Volume 31, Number 4, pp. 1301-1322

Published in 2017 Apr (Electronic publication in Aug. 4, 2016, midnight )

(Abstract) We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.
(MeSH Terms)

Contact
Toshihide Kobayashi , University of Strasbourg , Faculty of pharmacy , UMR 7021 CNRS
Contributors
Asami Makino, Mitsuhiro Abe, Reiko Ishitsuka, Motohide Murate, Takuma Kishimoto, Shota Sakai, Françoise Hullin-Matsuda, Yukiko Shimada, Takehiko Inaba, Hideyuki Miyatake, Hideko Tanaka, Atsushi Kurahashi, Chan-Gi Pack, Rinshi S. Kasai, Shuku Kubo, Nicole L. Schieber, Naoshi Dohmae, Naoya Tochio, Kyoji Hagiwara, Yutaka Sasaki, Yoko Aida, Fumihiro Fujimori, Takanori Kigawa, Kozo Nishibori, Robert G. Parton, Akihiro Kusumi, Yasushi Sako, Gregor Anderluh, Makoto Yamashita, Tetsuyuki Kobayashi, Peter Greimel, Toshihide Kobayashi

OMERO Dataset
OMERO Project
Source