Detail of fig10b-1



Project
Title
Near-infrared (NIR) fluorescene image of the breast tumor mouse injected by indocyanine green (ICG)-Ab containing S4-6 micelles for 0 hour
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
554.0 KB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
KPL-4
Cell Line
-

Datatype
breast tumor fluorescence
Molecular Function (MF)
Biological Process (BP)
naphthalenesulfonate metabolic process ( GO:0018984 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
-
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
FluorescenceMicroscope
Acquisition mode
FluorescenceCorrelationSpectroscopy
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Jin et al. (2016) Medchemcomm, 7(4): 623-631.
Related paper(s)

Jin, Takashi, Tsuboi, Setsuko, Komatsuzaki, Akihito, Imamura, Yukio, Muranaka, Yoshinori, Sakata, Takao, Yasuda, Hidehiro (2016), Enhancement of aqueous stability and fluorescence brightness of indocyanine green using small calix[4]arene micelles for near-infrared fluorescence imaging, Med. Chem. Commun., Volume 7, Number 4, 623-631

Published in 2016

(Abstract) Indocyanine green (ICG) is the only near-infrared (NIR) fluorescent dye which is approved for medical applications. However, ICG has several drawbacks such as aqueous instability, photodegradation, and low fluorescence quantum yield (2.5% in water), which lead to the limitation on the use of ICG for in vitro and in vivo NIR fluorescence imaging. Free ICG rapidly aggregates in physiological buffer solutions, and its fluorescence diminishes within several days. The objective of this work is to provide an easy method for the enhancement of the stability and fluorescence brightness of ICG in aqueous solutions for NIR fluorescence imaging. Herein, we report that the incorporation of ICG into small calix[4]arene (S4-6) micelles (<5 nm in diameter) significantly improves the aqueous stability and fluorescence brightness of ICG. The fluorescence quantum yields of ICG-calix[4]arene micelles are increased up to ∼6% in aqueous solutions. Using the ICG-calix[4]arene micelles, we achieved non-invasive NIR fluorescence imaging of the liver and lymph system in mice. Furthermore, we achieved NIR fluorescence imaging of nude mice bearing human breast tumors using an ICG conjugated antibody which is incorporated into the calix[4]arene micelles. Preparation of the calix[4]arene micelles including ICG is very easy and the micelle system does not show significant cytotoxicity. The ICG-calix[4]arene micelle system acts as a highly stable and bright probe for in vitro and in vivo NIR fluorescence imaging.

Contact
Takashi Jin , RIKEN , Quantitative Biology Center , Laboratory for Nano-Bio Probes
Contributors
Takashi Jin, Setsuko Tsuboi, Akihito Komatsuzaki, Yukio Imamura, Yoshinori Muranaka, Takao Sakata, Hidehiro Yasuda

OMERO Dataset
OMERO Project
Source