Detail of Sublingual_bioengineered



Project
Title
Phase contrast images of the ED14.5 bioengineered sublingual gland germs on days 0,1,2,3 organ cultures
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
33.5 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
generation of salivary gland
Molecular Function (MF)
Biological Process (BP)
salivary gland development ( GO:0007431 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 1.290 micrometer/pixel, Z: NA
T scale
24 hour for each time interval

Image Acquisition
Experiment type
Other
Microscope type
BrightfieldMicroscope
Acquisition mode
WideField
Contrast method
Phase
Microscope model
Carl Zeiss AxioCAM MRc5
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Ogawa et al. (2013) Nat Commun, 4: 2498.
Related paper(s)

Miho Ogawa, Masamitsu Oshima, Aya Imamura, Yurie Sekine, Kentaro Ishida, Kentaro Yamashita, Kei Nakajima, Masatoshi Hirayama, Tetsuhiko Tachikawa, Takashi Tsuji (2013) Functional salivary gland regeneration by transplantation of a bioengineered organ germ., Nature communications, Volume 4, pp. 2498

Published in 2013

(Abstract) Salivary gland hypofunction, also known as xerostomia, occurs as a result of radiation therapy for head cancer, Sjogren's syndrome or aging, and can cause a variety of critical oral health issues, including dental decay, bacterial infection, mastication dysfunction, swallowing dysfunction and reduced quality of life. Here we demonstrate the full functional regeneration of a salivary gland that reproduces the morphogenesis induced by reciprocal epithelial and mesenchymal interactions through the orthotopic transplantation of a bioengineered salivary gland germ as a regenerative organ replacement therapy. The bioengineered germ develops into a mature gland through acinar formations with a myoepithelium and innervation. The bioengineered submandibular gland produces saliva in response to the administration of pilocarpine and gustatory stimulation by citrate, protects against oral bacterial infection and restores normal swallowing in a salivary gland-defective mouse model. This study thus provides a proof-of-concept for bioengineered salivary gland regeneration as a potential treatment of xerostomia.
(MeSH Terms)

Contact
Takashi Tsuji , Tokyo University of Science , Research Institute for Science and Technology
Contributors
Miho Ogawa, Masamitsu Oshima, Aya Imamura, Yurie Sekine, Kentaro Ishida, Kentaro Yamashita, Kei Nakajima, Masatoshi Hirayama, Tetsuhiko Tachikawa, Takashi Tsuji

OMERO Dataset
OMERO Project
Source