Detail of fig2b_enamelknot



Project
Title
Time-lapse confocal images of the late developmental stage of a split tooth germ from a transgenic mouse embryo
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
80.7 GB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
tooth dynamics
Molecular Function (MF)
Biological Process (BP)
odontogenesis ( GO:0042476 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.55 micrometer/pixel, Z: 1.82 micrometer/slice
T scale
45 min for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM780
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Yamamoto et al. (2015) Sci Rep, 5: 18393.
Related paper(s)

Naomi Yamamoto, Masamitsu Oshima, Chie Tanaka, Miho Ogawa, Kei Nakajima, Kentaro Ishida, Keiji Moriyama, Takashi Tsuji (2015) Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field., Scientific reports, Volume 5, pp. 18393

Published in 2015 Dec 17 (Electronic publication in Dec. 17, 2015, midnight )

(Abstract) The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force.
(MeSH Terms)

Contact
Takashi Tsuji , RIKEN , Center for Biosystems Dynamics Research , Laboratory for Organ Regeneration
Contributors
Naomi Yamamoto, Masamitsu Oshima, Chie Tanaka, Miho Ogawa, Kei Nakajima, Kentaro Ishida, Keiji Moriyama, Takashi Tsuji

OMERO Dataset
OMERO Project
Source